| 不对称还原丙酮酸乙酯菌株的筛选及鉴定 |
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| 引用本文： | 王丹,李旺,巩春甫,杨四佳,张强,唐恺迪,戚南昌,银丽娟,张杰,杨志荣.不对称还原丙酮酸乙酯菌株的筛选及鉴定[J].应用与环境生物学报,2012(3):460-464. |
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| 作者姓名： | 王丹 李旺 巩春甫 杨四佳 张强 唐恺迪 戚南昌 银丽娟 张杰 杨志荣 |
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| 作者单位： | 四川大学生命科学学院;成都医学院生物医学系;成都医学院基础医学院;成都医学院检验医学院 |
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| 基金项目： | 四川省科技厅项目(No.2010GZ0164)资助~~ |
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| 摘 要： | 以丙酮酸乙酯为底物,从成都某化工厂污水池及其附近土壤中分离到36株可将丙酮酸乙酯不对称还原成(S)-乳酸乙酯的菌株.经过多次复筛,最终获得了一株具有较高催化活性的酵母菌BTY18-6.在以该菌株静息细胞为催化剂,催化不对称还原丙酮酸乙酯合成(S)-乳酸乙酯的反应中,底物浓度为60 mmol/L时,底物转化率为86.9%,产物(S)-乳酸乙酯的ee值为88.7%.通过对其形态学、生理生化特征及其26S rDNA Dl/D2区域的分析表明,BTY18-6为胶红酵母.
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| 关 键 词： | 丙酮酸乙酯 不对称还原 (S)-乳酸乙酯 静息细胞 26S rDNA D1/D2 胶红酵母 |
Screening and Identification of a Strain for Asymmetric Reduction of Ethyl Pyruvate |
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| WANG Dan,LI Wang,GONG Chunfu,YONG Sijia,ZHAGN Qiang,TANG Kaidi,QI Nanchang,YIN Lijuan,ZHANG Jie,& YANG Zhirong.Screening and Identification of a Strain for Asymmetric Reduction of Ethyl Pyruvate[J].Chinese Journal of Applied and Environmental Biology,2012(3):460-464. |
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| Authors: | WANG Dan LI Wang GONG Chunfu YONG Sijia ZHAGN Qiang TANG Kaidi QI Nanchang YIN Lijuan ZHANG Jie & YANG Zhirong |
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| Institution: | 1(1College of Life Sciences,Sichuan University,Chengdu 610065,China)(2Department of Biomedicine,Chengdu Medical College,Chengdu 610083,China)(3School of Basic Medicine,Chengdu Medical College,Chengdu 610083,China)(4School of Examination Medical,Chengdu Medical College,Chengdu 610083,China) |
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| Abstract: | Thirty-six strains exhibiting asymmetric reduction of ethyl pyruvate to(S)-ethyl lactate were isolated from sewage and the neighboring soil samples of a chemical factory in Chengdu,Sichuan,China.Among them,yeast strain BTY18-6 with high catalytic activities was screened.The asymmetric reduction of ethyl pyruvate to(S)-ethyl lactate by BTY18-6 resting cells in aqueous phase was examined.When the concentration of ethyl pyruvate was 60 mmol/L,the conversion rate could reach 86.9% with enantiomeric excesses(ee) of 88.7%.Based on the analysis of physiological and biochemical characteristics and 26S rDNA D1/D2 domain sequence with its general morphology,BTY18-6 was identified as Rhodotorula mucilaginosa. |
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| Keywords: | ethyl pyruvate asymmetric reduction (S)-ethyl lactate resting cell 26S rDNA D1/D2 Rhodotorula mucilaginosa |
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