多拷贝表达转鲑鱼降钙素基因酵母的急性、亚急性毒性

口服转鲑鱼降钙素基因酵母为鲑鱼降钙素的应用开辟了一条新的途径.为评价转鲑鱼降钙素基因酵母的安全性,将转基因酵母分别灌胃昆明种小鼠和Wistar大鼠进行急性毒性实验(7 d)、亚急性毒性实验(8周).急性毒性实验结果表明灌喂转基因酵母对小鼠无致死效应(LD50>10 000 mg/kg);亚急性毒性实验中高(2.0 g/kg)、低(0.5 g/kg)剂量组动物的一般状况、体重、主要脏器系数、血液学指标及血液生化指标与各对照组比较,均未见明显差异(P>0.05),组织病理切片检查未发现病理性变化.实验结果说明转鲑鱼降钙素基因酵母不会对实验动物造成不良影响,是安全无毒的.图3表3参16

Acute and Subacute Toxicity of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin

Oral administration recombinant Saccharomyces cerevisiae expressing salmon calcitonin made a new method for salmon calcitonin delivery system.Transgenic S.cerevisiae was evaluated for its acute(7 days) and subacute toxicity(8 weeks) by the oral route in KM mouse and Wistar rats respectively.In the acute toxicity test,every group did not produce any hazardous symptoms or deaths(LD50> 10 000 mg/kg).The subacute treatment with transgenic S.cerevisiae(2.0 and 0.5 g/ kg) failed to change body weight gain,food and water consumption.Organ/weight ratios,haematological and biochemical indices analysis showed no significant differences(P > 0.05) in any of the parameters examined.There was no pathological change observed in slice.These results suggested that recombinant S.cerevisiae expressing salmon calcitonin had no negative influences on the tested animal.It was safe and nontoxic.Fig 3,Tab 3,Ref 16