| 绿僵菌钙传感蛋白基因克隆及其与致病性的关系 |
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| 引用本文： | 李敏,曹月青.绿僵菌钙传感蛋白基因克隆及其与致病性的关系[J].应用与环境生物学报,2012(2):240-244. |
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| 作者姓名： | 李敏 曹月青 |
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| 作者单位： | 重庆大学生物工程学院基因工程中心,重庆市杀虫真菌生物农药工程中心 |
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| 基金项目： | 国家自然科学基金项目(N o.30800736);中央高校基本科研业务费(No.CDJRC10230008)资助~~ |
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| 摘 要： | 通过筛选文库获得绿僵菌钙传感蛋白基因MaNcs1基因全长cDNA序列,并对其在致病过程中的作用进行了分析.结果显示,MaNcs1基因cDNA全长792 bp,开放阅读框为573 bp,编码190个氨基酸;采用RNA干扰技术下调该基因的表达后,对东亚飞蝗的生测实验表明,绿僵菌毒力显著下降,说明该基因与绿僵菌的致病性有关,为以后深入研究该基因的致病机制奠定了基础.
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| 关 键 词： | 绿僵菌 MaNcs1 钙传感蛋白 RNA干扰 致病性 毒力 |
Cloning of MaNcs1 Gene and Its Role in Pathogenicity of Metarhizium acridum |
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| LI Min & CAO Yueqing.Cloning of MaNcs1 Gene and Its Role in Pathogenicity of Metarhizium acridum[J].Chinese Journal of Applied and Environmental Biology,2012(2):240-244. |
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| Authors: | LI Min & CAO Yueqing |
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| Institution: | LI Min & CAO Yueqing(Genetic Engineering Research Center,Institute of Bioengineering,Chongqing University;Chongqing Engineering Research Center for Fungal Insecticides,Chongqing 400044,China) |
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| Abstract: | MaNcs-1 gene was cloned by screening the cDNA library of Metarhizium acridum,and its role in the pathogenicity of M.acridum was also investigated.The results showed that the MaNcs1 cDNA had 792 bp in full length containing an open reading frame(ORF) of 573 bp,which encoded a protein with 190 amino acid residues.Compared with the wild type strain,the virulence of M.acridum against Locusta migratoria manilensis(Meyen) was significantly decreased when the MaNcs1 gene was down-regulated using RNA interference,indicating that the MaNcs1 gene was involved in the pathogenesis of M.acridum.These results would contribute to the further study in depth on its pathogenic mechanism in M.acridum. |
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| Keywords: | Metarhizium acridum MaNcs1 calcium sensor NCS-1 gene RNA interference pathogenicity virulence |
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