| 硫自养高氯酸盐还原菌培养与驯化 |
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| 引用本文： | 高孟春,赵从从,张健,王子超,孙若晨,任云,李亚惠.硫自养高氯酸盐还原菌培养与驯化[J].环境工程学报,2014,8(4):1257-1261. |
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| 作者姓名： | 高孟春 赵从从 张健 王子超 孙若晨 任云 李亚惠 |
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| 作者单位： | 1. 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100;2. 中国海洋大学环境科学与工程学院, 青岛 266100 |
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| 基金项目： | 国家自然科学基金资助项目（21077096）；中国海洋大学国家大学生创新训练项目（201210423090） |
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| 摘 要： | 考察了以污水处理厂的好氧污泥作为接种污泥,以S0作为电子供体培养驯化硫自养高氯酸盐还原菌的过程,并利用PCR-DGGE技术对不同阶段的污泥样品进行群落分析。结果表明,接种污泥在经过短暂的适应期后能具有较高的还原ClO-4的性能,随着驯化时间的延长,降解速率显著提高。培养驯化过程中微生物种群结构发生了演替变化,菌种Dechloromonas sp.CL、Quadrisphaera granulorum、Comamonadaceae bacterium 32-4、Acidovorax caeni、filamentous bacterium Plant1Iso8、Candidatus Nitrospira defluvii存在整个驯化培养阶段。随着培养驯化时间的延长,菌种Bacteroidetes bacterium S22-33、Herbaspirillum huttiense逐渐消失,出现新菌种Methyloversatilis universalis、beta proteobacterium HTCC379、beta proteobacterium CDB21和Clostridium bifermentans,并逐渐成为优势菌种。
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| 关 键 词： | 高氯酸盐 硫自养高氯酸盐还原菌 群落结构 PCR-DGGE |
| 收稿时间： | 3/9/2013 12:00:00 AM |
Enrichment and cultivation of sulfur autotrophic perchlorate-reducing consortium |
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| Gao Mengchun,Zhao Congcong,Zhang Jian,Wang Zichao,Sun Ruochen,Ren Yun and Li Yahui.Enrichment and cultivation of sulfur autotrophic perchlorate-reducing consortium[J].Techniques and Equipment for Environmental Pollution Control,2014,8(4):1257-1261. |
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| Authors: | Gao Mengchun Zhao Congcong Zhang Jian Wang Zichao Sun Ruochen Ren Yun and Li Yahui |
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| Institution: | 1. Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2. College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China |
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| Abstract: | Enrichment and cultivation of sulfur autotrophic perchlorate-reducing consortium were investigated by using aerobic sludge from sewage plant and sulfur as seeding sludge and electron donor,respectively.The seeding sludge had higher perchlorate removal efficiency after a short adaptation and the removal efficiency gradually increased during the enrichment and cultivation.ClO4- could completely transform to Cl-.The successional change of microbial community was also investigated by PCR-DGGE technique.Some strains existed during the whole enrichment and cultivation,such as Dechloromonas sp.CL,Comamonadaceae bacterium 32-4,Quadrisphaera granulorum,Acidovorax caeni,filamentous bacterium Plant1 Iso8 and Candidatus Nitrospira defluvii.With the time increasing of enrichment and cultivation,Bacteroidetes bacterium S22-33 and Herbaspirillum huttiense disappeared,and Methyloversatilis universalis,beta proteobacterium HTCC379,beta proteobacterium CDB21 and Clostridium bifermentans appeared as new species,which gradually became the predominant strains. |
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| Keywords: | perchlorate sulfur autotrophic perchlorate-reducing consortium community structure PCR-DGGE |
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