4株正十六烷降解菌的降解能力及代谢动力学特性

从处理采油废水的活性污泥中分离出4株产生物表面活性剂的正十六烷高效降解菌。菌株A14和B45为非脱羧勒克菌（Leclercia adecarboxylata），菌株C28和A27为肠杆菌（Enterobacter sp.）。在NaCl质量浓度15~25 g/L、pH 6.0~7.0、接种量10%（φ），培养温度37 ℃，摇床转速160 r/min、正十六烷体积分数0.30%的条件下降解16 d后，菌株A14、B45、C28和A27的正十六烷降解率分别为93.7%，87.8%，73.3%，65.7%。4株菌所产生的生物表面活性剂均为磷脂类表面活性剂。菌体生长满足逻辑斯蒂模型，正十六烷的降解满足一级反应动力学模型。菌株C28、A27的生长速率快于菌株A14、B45，菌株A14、B45的正十六烷降解速率快于菌株C28、A27。

Degradation capabilities and metabolic kinetics characteristics of 4 strains of n-hexadecane degrading bacteria

Four strains of high-efficiency n-hexadecane degrading bacteria with biosurfactant production were isolated from activated sludge in treatment of oilfield wastewater. Strain A14 and B45 were identified as Leclercia adecarboxylata，while strain C28 and A27 were identified as Enterobacter sp.. Under the conditions of NaCl mass concentration 15-25 g/L，pH 6.0-7.0，inoculation quantity 10%（φ），culture temperature 37 ℃，rotation speed 160 r/min，n-hexadecane volume fraction 0.30% and degradation time 16 d，the degradation rate by strain A14，B45，C28 and A27 was 93.7%，87.8%，73.3% and 65.7%，respectively. All the biosurfactants produced by the 4 strains were phospholipid surfactants. The growth of the strains followed the Logistic model，and the degradation of n-hexadecane followed the first order reaction kinetics model. The growth rate of strain C28 and A27 was faster than that of strain A14 and B45，however，the degradation rate by strain A14 and B45 was faster than that of strain C28 and A27.