Effects of the inhibitor of pyruvate dehydrogenase multi-enzyme complex on hydrogen production by fermentative microbes |
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| Authors: | Zhao-xia Song Hong-wu He Yao-ting Fan Hong-wei Hou |
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| Institution: | 1. College of Resources and Environment, He’nan University of Engineering, Zhengzhou, China;2. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China;3. Key Laboratory of Pesticide and Chemical Biology of the Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, China;4. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China |
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| Abstract: | The redox state of carbon sources directly affected the ratio of NADH/NAD+ which was coupled to the hydrogen production by Bacillus sp. FS2011. The addition of the inhibitor of pyruvate dehydrogenase multi-enzyme complex (PDHc)E1 could regulate hydrogen production by FS2011 or pretreated compost in batch cultivation. With the addition of appropriate amount of inhibitor, hydrogen production via the NADH pathway was increased, leading to the higher overall hydrogen production. The maximum hydrogen yields of 307.6 ± 13.21 mL/g by FS2011 with the inhibitor of 80 ppm and 362.1 ± 10.1 mL/g by pretreated compost with the inhibitor of 60 ppm were observed, which were increased by 8.7% and 17.8% compared with the controls, respectively. Meanwhile the production of soluble metabolic byproducts such as butyrate, acetate and so on were decreased, resulting in reducing the difficulty of wastewater treatment. |
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| Keywords: | Bacillus sp FS2011 hydrogen production inhibitor NADH/NAD+ ratio pretreated compost |
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