间作与接种AMF对连作土壤微生物群落结构与功能的影响

为了探明间作种植模式和接种AMF对连作土壤的影响,试验选取马铃薯连作10年土壤为基质,采用两因素随机区组盆栽实验设计,研究间作及接种AMF对连作土壤微生物群落结构及功能的影响。结果表明：在不同间作模式及AMF接种后,连作土壤微生物群落结构、功能均有不同程度的差异。间作调控后马铃薯｜｜玉米｜｜蚕豆处理土壤细菌比例最高,放线菌次之,真菌最低;Biolog Eco板平均颜色变化率（AWCD）比对照高36.77%。间作并接种AMF（Gloumus etunicatum）后,土壤微生物功能多样性增强,土壤真菌的比例上升,细菌、放线菌的比例下降;马铃薯｜｜蚕豆、马铃薯｜｜玉米｜｜蚕豆土壤孢子密度与其他处理有显著差异（P〈0.05）;马铃薯｜｜玉米｜｜蚕豆处理在培养96 h后的平均颜色变化率（AWCD）值比单作高48.96%。各处理土壤根际微生物群落在2种方式的调控下对碳水化合物、氨基酸碳源利用强度较高,对芳香化合物的利用能力较弱;调控后碳源利用类型开始从碳水化合物、胺类化合物向胺类化合物和多聚化合物利用类型转变。间作调控和AMF调控对土壤微生物群落功能多样性的增加可能存在一定的叠加效应。土壤微生物群落结构、功能多样性的变化是多种因子综合作用的结果。

Effects of intecropping and inoculation of AMF on the microbial community structure and function of continuous cropping soil

In order to comprehend the effects of intercropping and inoculation of AMF on the continuous cropping soil, we selected the soil from the south of Ningxia region which has been continuous cropping for ten years as the substace, and used the two factor randomized block pot experimental design to study the effects of intercropping and inoculation of AMF on its soil microbial community structure and function. The results showed： After taking the control of intercropping and inoculation of AMF, the microbial community structural and functional diversity had some certain differences. Under the regulation of intercropping, the percentage of bacteria in potato/maize/broad bean was the highest, actinomycetes followed, and fungus lowest; The AWCD was 36.77% higher than CK. After inoculated AMF（G. etunicatum）, the microbial functional diversity increased, the percentage of fugus was increased, bacteria and attinomycetes dropped; The spore density in potato/broad bean and potato/maize/broad bean had a significant difference with other treatments（P〈0.05）; The AWCD in potato/maize/broad bea increased 48.96% than CK. The soil microorganisms in all treatments under this two controls had a greater usage intensity of carbon compound and amino acid, and used the aromatic compound lest ; The carbon resource utilization patter changed from carbon compound, amine compound to amine compound and polymer compound after the regulation. And maybe there＇s an over!apping effects between the regulation of intercropping and AMF on the improvement of the soil microbial community structural and functional diversity which was combined affected by multi-factors.