水质净化高效复合微生态制剂的研制

在室内模拟条件下,采用正交实验的方法对光合细菌、枯草芽孢杆菌和反硝化细菌的复配比例进行了研究,筛选一种用于水产养殖水质净化的高效复合微生态制剂。结果表明,当光合细菌(菌细胞浓度约为2×109CFU/mL)、枯草芽孢杆菌(菌细胞浓度约为8×108CFU/mL)和反硝化细菌(菌细胞浓度约为8×108CFU/mL)按菌液体积比为1∶2∶1进行复配利于水中溶解氧的提高和COD、氨氮、亚硝态氮、硝态氮的降解。验证实验表明,筛选组合各指标均优于商品微生态制剂和空白对照,其中溶解氧含量显著高于商品微生态制剂EM和复合芽孢菌处理,在实验第5天对COD的降解率为95%,显著优于EM和复合芽孢菌处理的66.3%和47.9%,实验第7天对氨氮、亚硝态氮和硝态氮的降解率分别达到70%、89%和56%。

Development of high efficiency compound probiotics for water purification

The orthogonal-design experiment was applied to screen optimal mixed proportion of photosynthesis bacteria(Rhodopseudomonas sp.),Bacillus subtilis and denitrifying bacteria(pseudomonas sp.) under the simulated conditions to get a high efficiency compound probiotics for aquaculture water purification.The results showed that the optimal combination of photosynthesis bacteria(2×109 CFU/mL),Bacillus subtilis(8×108 CFU/mL) and denitrifying bacteria(8×108 CFU/mL) was 1∶2∶1 in volume which in favor of dissolved oxygen increasing and COD,ammonia nitrogen,nitrite and nitrate removal.The validation test results indicated that the water could be effectively purified by the compound probiotics and the effect exceeded the EM,complex bacillus and control,such as the dissolved oxygen content of the treatment was significantly higher than that of the EM and complex bacillus.At the 5th day the degradation rate of COD was 95%,significantly better than that of the EM(66.3%) and complex bacillus(47.9%),at the 7th day the ammonia nitrogen,nitrite and nitrate were reduced by up to 70%、89% and 56%,respectively.