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| He-Ne激光诱变选育高效石油烃降解菌的研究 | |
| 引用本文： | 张子间,刘勇弟,卢杰,张立辉.He-Ne激光诱变选育高效石油烃降解菌的研究[J].环境工程学报,2012,6(2):677-682. |
| 作者姓名： | 张子间 刘勇弟 卢杰 张立辉 |
| 作者单位： | 1. 华东理工大学资源与环境工程学院,上海200237／山东理工大学资源与环境工程学院,淄博255049 2. 华东理工大学资源与环境工程学院,上海,200237 3. 山东理工大学资源与环境工程学院,淄博,255049 |
| 基金项目： | 国家自然科学基金资助项目(30600119) |
| 摘 要： | 采用He-Ne激光器对绿针假单胞菌(Pseudomonas chlororaphis)进行激光诱变育种。在激光照射功率10 mW,时间10 min条件下,筛选到一株遗传性状稳定的高效石油烃降解菌PS 2。摇瓶实验发现当培养液中初始柴油含量为0.2%～0.5%(V/V)、温度为30℃左右、pH值为7～8的条件下,突变菌PS 2对石油烃的降解效果最好。在最适生长条件下,突变菌PS 2在120 h内将培养液中的石油烃完全降解且不存在延滞期,比出发菌株少用24 h。结果表明,He-Ne激光诱变育种技术是获得高效石油烃降解菌的有效途径之一。 |
| 关 键 词： | 绿针假单胞菌 He-Ne激光诱变 遗传稳定性 |
Study on screening of a highly petroleum hydrocarbon-degrading bacteria by He-Ne laser induced mutation |
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| Zhang Zijian,Liu Yongdi,Lu Jie and Zhang Lihui.Study on screening of a highly petroleum hydrocarbon-degrading bacteria by He-Ne laser induced mutation[J].Techniques and Equipment for Environmental Pollution Control,2012,6(2):677-682. | |
| Authors: | Zhang Zijian Liu Yongdi Lu Jie and Zhang Lihui |
| Institution: | 1(1.School of Resources and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China; 2.School of Resources and Environmental Engineering,Shandong University of Technology,Zibo 255049,China) |
| Abstract: | The Pseudomonas chlororaphis was irradiated at 10 mW for 10 minutes using He-Ne laser.A mutant PS 2 with steady genetic characteristics and high degradation rate of petroleum hydrocarbon was obtained.The result showed that degradation efficiency is satisfactory when the content of petroleum hydrocarbon is 0.2%~0.5%(V/V),temperture is about 30℃,pH is 7~8 in the batch culture experiment.Under these conditions for optimum growth,petroleum hydrocarbon could be completely degraded by mutant PS 2 within 120 h without lag phase.The results showed that laser inducing was one of effective ways for screening excellent petroleum hydrocarbon-degrading strains. |
| Keywords: | Pseudomonas chlororaphis He-Ne laser mutation heritance stability |
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