| 1,1-二氯乙烯降解菌的分离鉴定及降解特性 |
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| 引用本文： | 周雪,唐顺,李冬梅,杨琦,尚海涛,郝春博.1,1-二氯乙烯降解菌的分离鉴定及降解特性[J].环境工程学报,2010,4(5):983-987. |
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| 作者姓名： | 周雪 唐顺 李冬梅 杨琦 尚海涛 郝春博 |
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| 作者单位： | 1. 中国地质大学(北京)水资源与环境学院,北京,100083
2. 中国地质大学(北京)水资源与环境学院,北京100083;中国地质大学(北京)地质微生物实验室,北京100083 |
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| 基金项目： | 国家自然科学基金资助项目（40102027，50578151）；北京市自然科学基金资助项目（8052017，8092025）；北京市教委产学研项目（51900265005）；国家水体污染控制与治理重大专项资助（2009ZX07419）；国家科技重大专项（2009ZX07207-008，2009ZX07419-002，2009ZX07207-001） |
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| 摘 要： | 从好氧活性污泥中分离得到一株能以1,1-二氯乙烯(1,1-DCE)作为惟一碳源和能源生长的革兰氏阴性菌株D-B,经鉴定属于产碱杆菌属(Alcaligenessp.)。当维持菌株D-B浓度一定时,1,1-DCE的去除率随着1,1-DCE浓度的增大先增加后降低,且降解过程主要发生在加入1,1-DCE后的3~5 h内。当1,1-DCE的初始浓度为300μg/L时去除率达到最大值85.32%。菌株D-B对1,1-DCE的降解符合Monod方程,饱和常数Ks=21.96 mg/L,1,1-DCE的最大比基质降解速率Vmax=50.76 mg/(L.h)。
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| 关 键 词： | 1 1-二氯乙烯 生物降解 分离鉴定 动力学 Monod方程 |
Isolation and identification of a 1,1-dichloroethylene-degrading bacterial strain and its degradation characteristics |
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| Zhou Xue,Tang Shun,Li Dongmei,Yang Qi,Shang Haitao and Hao Chunbo.Isolation and identification of a 1,1-dichloroethylene-degrading bacterial strain and its degradation characteristics[J].Techniques and Equipment for Environmental Pollution Control,2010,4(5):983-987. |
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| Authors: | Zhou Xue Tang Shun Li Dongmei Yang Qi Shang Haitao and Hao Chunbo |
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| Institution: | 1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China,1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China,1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China,1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China,1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China and 1.School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China; 2.Laboratory of Geomicrobiology, China University of Geosciences, Beijing 100083, China |
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| Abstract: | A Gram-negative bacterial strain D-B,which was able to grow on 1,1-dichloroethylene(1,1-DCE)as sole carbon and energy source,was isolated from aerobic activated sludge and identified as Alcaligenes sp.When maintaining the same concentration of strain D-B,the biological removal rate of 1,1-DCE increased at first and then decreased with the increasing 1.1-DCE and the degradation was mainly taken place within 3 to 5 hours after adding 1.1-DCE.The removal rate of 1.1-DCE attained the maximum value of 85.32% when the initial concentration of 1,1-DCE was 300 μg/L.The removal of 1,1-DCE degraded by strain D-B was in accordance with the Monod equation:the saturation constant was K_s=21.96 mg/L and the maximum specific substrate degradation rate of 1,1-DCE was V_(max)=50.76 mg/(L·h). |
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| Keywords: | 1 1-dichloroethylene biodegradation isolation and identification kinetics Monod equation |
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