Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli |
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| Authors: | Satoshi SUZUKI Norikazu MATSUMURA Takeshi OHOKA Shun SAKUMA Tomohiro NAKAHATA Masahide ISHIKAWA |
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| Institution: | 1. Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan;2. Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan;3. Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, China;1. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University (BUAA), Beijing 100191, China;2. State Key Laboratories of Transducer Technology, National Engineering Lab for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;1. Department of Chemistry, Rutgers University, Newark, NJ 07102, United States;2. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, University Heights, Newark, NJ 07102, United States;1. Free Radical Group, The Heart Research Institute, Newtown, NSW 2042, Australia;2. Faculty of Medicine, University of Sydney, Sydney, NSW 2006, Australia;1. State Key Laboratories of Transducer Technology, National Engineering Lab for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;4. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University (BUAA), Beijing 100191, China |
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| Abstract: | Enzymes fixed on the electrode of biosensor are gradually inactivated and the electrode is discarded after using several times. In order to prepare the stable biosensor, we try to use a stable enzyme from extreme thermophilic bacteria, Thermus thermophilus HB8. It is very important that a stable enzyme from T. thermophilus HB8 is overproduced in Escherichia coli, for the purpose of enough supply of enzyme. Thereby, we determined the important sequence for overexpression of NADH oxidase (nox) gene from T. thermophilus HB8 in E. coli. As a result, it is revealed that ten nucleotides sequence, GAAATTAACT, in the upstream of start codon of nox gene was important for its overexpression in E. coli. |
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