邻苯二甲酸二丁酯(DBP)与卵清白蛋白(OVA)联合染毒对小鼠肺脏和脾脏组织氧化应激的作用

为研究邻苯二甲酸二丁酯(dibutylphthalate,DBP)单独染毒及与卵清白蛋白(ovalbumin,OVA)联合染毒对小鼠肺脏和脾脏组织氧化应激的作用,将BALB/c小鼠随机分为8组:(1)未处理对照组(生理盐水组);(2)0.5mg·kg-1DBP染毒组;(3)5.0mg·kg-1DBP染毒组;(4)50mg·kg-1DBP染毒组;(5)1.67mg·kg-1OVA单独染毒组;(6)0.5mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组;(7)5.0mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组;(8)50mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组。未处理对照组和DBP染毒组每天按体质量给予生理盐水和DBP灌胃。2周后,测定肺脏组织活性氧物种(ROS)、谷胱甘肽(GSH)和丙二醛(MDA)含量以及脾脏组织ROS、GSH含量。结果显示,联合染毒组相较于其他组的肺脏组织各指标均有不同的显著性差异(P<0.05),联合染毒组的脾脏组织中ROS含量较其他组有显著差异(P<0.05),而GSH含量无统计学意义(P>0.05)。结果说明,DBP与OVA联合染毒能够增强肺脏组织的氧化应激作用,对于脾脏组织的氧化应激作用不明显;DBP在联合染毒中显示一定免疫佐剂效应。

Oxidative Stress Induced by Combined Exposure of Dibutyl Phthalate (DBP) and Ovabumin (OVA) in the Lung and Spleen of Mice

To study the oxidative stress in the lung and spleen of mice from dibutyl phthalate (DBP) alone or conbined with ovalbumin (OVA), the BALB/c mice were randomly divided into eight groups: (1) untreated control (saline) group; (2) 0.5 mg·kg^-1 DBP exposure group; (3) 5.0 mg·kg^-1 DBP exposure group; (4) 50 mg·kg^-1 DBP exposure group;(5) OVA exposure group; (6) 0.5 mg·kg^-1 DBP and OVA combined exposure group; (7) 5.0 mg·kg^-1 DBP and OVA combined exposure group; (8) 50 mg·kg^-1 DBP and OVA combined exposure group. The untreated control and DBP exposure groups were given saline and DBP to gavage calculated by body mass everyday. After two weeks, it was measured that the ROS, GSH and MDA contents in the lung tissue and the ROS and GSH contents in the spleen tissue of mice. The results showed that the lung tissue's biomarkers and the spleen's ROS of DBP and OVA combined exposure groups have significant different (P<0.05) compareh with other groups, whereas the content of GSH has no statistically significant difference (P>0.05). It is suggested that DBP and OVA combined exposure can enhance the oxidative stress in lung tissues, but the oxidative stress in the spleen tissues is not obvious. DBP exhibited certain immune adjuvant effect in combined exposure group.