聚乙烯醇与海藻酸钠对玫瑰色微球菌的固定化及其脱氮性能的优化

为了提高玫瑰色微球菌(Rosy Micrococcus)的脱氮效果,利用聚乙烯醇(polyvinyl alcohol,PVA)、海藻酸钠(sodium alginate,SA)以及PVA+SA复合物对其进行固定包埋。应用正交与均匀设计法对固定化小球进行脱氮优化。结果表明,单独利用PVA与SA进行固定脱氮时,脱氮率最高分别达到29%与25%,而利用PVA+SA进行复合固定时,成球性能与脱氮率均优于单因子固定。复合固定脱氮的最优条件为:PVA 7.2%,SA 3%,CaCl2 3%,温度31℃,pH 7.2,胶菌比15%,小球数300个,脱氮时间48 h后脱氮率最高,达到48.9%。

Polyvinyl alcohol and sodium alginate immobilizing Rosy Micrococcus and optimization of nitrogen removal performance

In order to improve nitrogen removal effect of Rosy Micrococcus, PVA(polyvinyl alcohol), SA(sodium alginate), PVA+SA complexes were used as immobilized materials.Orthogonal and uniform design method were applied for the optimization of nitrogen removal performance in immobilized beads. Individually used PVA and SA as immobilized materials, the nitrogen removal rate reached 29% and 25%, respectively. However, PVA+SA complexes were used as immobilized materials, the pelletizing performance and nitrogen removal rates were superior to single factor fixation. The optimal conditions for compound immobile nitrogen removal were as follows:PVA7.2%, SA3%, CaCl23%, 31℃, pH 7.2, the proportion of the bacterial suspension and gel 15%, the number of small balls of 300, and under these conditions, the nitrogen removal rate reached 48.9% with nitrogen removal time of 48 h.