| 一株除草剂氰氟草酯降解菌的分离鉴定与降解特性 |
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| 引用本文： | 聂志娟,顾谢军,蔡舒,谢香婷,何健,郑金伟,李顺鹏.一株除草剂氰氟草酯降解菌的分离鉴定与降解特性[J].应用与环境生物学报,2011(6):869-875. |
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| 作者姓名： | 聂志娟 顾谢军 蔡舒 谢香婷 何健 郑金伟 李顺鹏 |
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| 作者单位： | 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室;金坛市环境监测站;南京农业大学资源与环境科学学院; |
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| 基金项目： | 国家自然科学基金项目(No.30970099); 农业部技术成果转化基金项目(No.2009GB23600516)资助~~ |
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| 摘 要： | 氰氟草酯(Calofop-buty1)是一种芳氧苯氧羧酸酯类除草剂,其迁移、转化和分解等行为对环境及人类健康具有潜在危害.为了解其微生物代谢机制,从长期使用氰氟草酯的稻田土壤中分离到1株氰氟草酯降解菌株,命名为QDZ-B.菌株QDZ-B的16S rDNA基因序列长度为1 400 bp,在RDP数据库比对结果表明其与寡养食单胞菌属菌株的同源性最近,与模式菌株嗜麦寡养食单胞菌Stenotrophomonas maltophilia(AB008509)的同源性为99.8%.根据表型特征、生理生化特性和16S rDNA序列同源性分析,将菌株QDZ-B鉴定为寡养单胞菌属(Stenotrophomonas sp.).接种量为5%时,菌株QDZ-B在基础盐液体培养基中5 d对100 mg L-1氰氟草酯的降解率约为88.2%.菌株QDZ-B的降解效果与接种量成正相关,但接种量大于5%时,降解效率趋于平稳.菌株QDZ-B降解氰氟草酯的最适温度为30℃,最适pH为7.0.通过质谱分析鉴定了3个菌株QDZ-B降解氰氟草酯的代谢产物,分别为氰氟草酸、氰氟草酰胺和2-氟-3-对-乙氧基苯氧基苯酰胺.
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| 关 键 词： | 氰氟草酯 除草剂 微生物降解 寡养单胞菌属 降解产物 |
Isolation of a Cyhalofop-butyl-degrading Bacterium and Its Degradation Characteristics |
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| NIE Zhijuan,GU Xiejun,CAI Shu,XIE Xiangting,HE Jian,ZHENG Jinwei & LI Shunpeng.Isolation of a Cyhalofop-butyl-degrading Bacterium and Its Degradation Characteristics[J].Chinese Journal of Applied and Environmental Biology,2011(6):869-875. |
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| Authors: | NIE Zhijuan GU Xiejun CAI Shu XIE Xiangting HE Jian ZHENG Jinwei & LI Shunpeng |
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| Institution: | NIE Zhijuan1,GU Xiejun3,CAI Shu1,XIE Xiangting1,HE Jian1,ZHENG Jinwei2 & LI Shunpeng1(1College of Life Sciences,Nanjing Agriculture University,Key Laboratory of Microbiological Engineering of Agricultural Environment,Nanjing 210095,China)(2College of Resources and Environmental Sciences,China)(3Environmental Monitoring Station of Jintan,Jintan 213200,Jiangsu,China) |
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| Abstract: | Cyhalofop-butyl(CyB) is a widely used aryloxyphenoxy propanoate(AOPP) herbicide for weed control in rice fields.Although herbicides are typically applied to the crop and weed canopy,substantial quantities of the active ingredient penetrate to the soil surface.Therefore,great concerns have been raised about the occurrence and fate of the herbicides in the environment.A cyhalofop-butyl-degrading bacterium strain,QDZ-B,was isolated from rice field soil that had been treated with cyhalofop-butyl for many years,... |
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| Keywords: | cyhalofop-buty1 herbicide biodegradation Stenotrophomonas sp metabolite product |
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