耐高盐撕裂蜡孔菌P2对模拟橙黄G染料废水的脱色及废水脱色前后的毒性测试

为评价撕裂蜡孔菌P2处理橙黄G染料废水的应用潜力,采用批次实验在开敞系统中研究静置与摇动、染料初始浓度、pH、温度、盐度、碳源、氮源、金属离子等因子对该菌降解橙黄G染料废水的影响,同时利用植物萌发与微生物抑菌试验进行染料与脱色溶液的毒性测试.结果表明,与摇动培养相比,静置培养更适合于撕裂蜡孔菌的脱色,最适脱色pH与温度分别为9和25℃.盐度测试结果显示撕裂蜡孔菌能在浓度为128 g L-1的盐溶液中能进行高效脱色,可达70%以上.在上述参数体系的优化基础上,分别进行了碳源、氮源与金属离子的添加优化实验,结果显示碳源、氮源与金属离子的最适浓度分别为4 g L-1葡萄糖、0.15 g L-1硝酸铵和0.1 mmol L-1 Zn2+.菌丝吸附在整个脱色过程中作用较小,撕裂蜡孔菌对橙黄G的脱色过程以酶的降解为主,未发现该菌分泌漆酶,只分泌锰过氧化物酶与木质素过氧化物酶,其最高活性分别为230 U mL-1和158 U mL-1.植物与微生物毒性分析显示撕裂蜡孔菌脱色后的产物对植物与微生物的毒性大大降低.因此,撕裂蜡孔菌对于处理橙黄G染料废水具有良好的应用潜力.

Toxicity Test and Decolorization of Simulated Orange G Dye Wastewater by Ceriporia lacerata P2 with a High-salinity Tolerance

In order to evaluate the application potential of Ceriporia lacerate P2 in treating dye wastewater,batch experiments were carried out in an air-opened system to investigate the effect of static and shaken culture,initial dye concentration,pH,temperature,salinity,carbon source,nitrogen source and metal ion on the decolorization of orange G dye wastewater by strain P2.Meanwhile,the toxicity test for the non-decolorizing dye and decolorizing products using plant seed germination method and antimicrobial test w...