发光细菌毒性测试条件的优化与毒性参照物的应用

针对传统发光细菌毒性测试方法中存在的结果重现性不高、使用剧毒物质氯化汞作为毒性参照物容易造成环境污染等问题,通过一系列实验优化了发光细菌生物毒性测试的条件:适宜pH值为5 0～9 0,最佳暴露时间为20min。同时筛选出了Zn2+作为毒性参照物代替传统方法中的剧毒物质HgCl2。Zn2+具有毒性中等、结果稳定、价格便宜等诸多优点。而且Zn2+与HgCl2的毒性之间存在一定相关性:Zn2+毒性为HgCl2毒性的1 12 5。采用Zn2+作为参照物不仅可以方便地表征不同化学物质的毒性,而且可以直观地表征复杂环境样品的毒性,是一种简便可行的方法。 

Optimization of Luminescent Bacteria Toxicity Test and Application of Toxicity Reference Substance

In the traditional luminescent bacteria toxicity test,the test result is not stable and the use of virulent substance HgCl2 as the toxicity reference substance engenders environmental pollution.In order to solve these problems,the experimental conditions were optimized by a series of experiments and the results indicated that the pH value of the samples should be adjusted to 5.0～9.0 and the best exposure time was 20 min.At the same time,zinc ion was selected as a toxicity reference substance to replace HgCl2.The advantages of using Zn2+ as the reference substance are less environmental pollution,more stable result and lower price,etc.Furthermore,there is a quantitative relationship between the toxicity of Zn2+ and the toxicity of HgCl2.The toxicity ratio of Zn2+ to HgCl2 is about 1/12.5.Using Zn2+ as the toxicity reference substance is a convenient and feasible method to represent the toxicity of both chemicals and complex environmental samples.