| 苯并[a]芘降解菌SL-1的筛选、鉴定及其降解特性 |
| |
| 引用本文： | 李艳秋,刘君,高鹏,刘慧军,孙建光,赵吉,胡海燕,屹立.苯并[a]芘降解菌SL-1的筛选、鉴定及其降解特性[J].内蒙古环境科学,2010,22(2):74-77. |
| |
| 作者姓名： | 李艳秋 刘君 高鹏 刘慧军 孙建光 赵吉 胡海燕 屹立 |
| |
| 作者单位： | 内蒙古煤炭建设生态环境研究院;中国农业科学院资源区划所;内蒙古大学生命科学学院; |
| |
| 基金项目： | 内蒙古自然科学基金(200508010604) |
| |
| 摘 要： | 从土壤中筛选出一株以苯并a]芘为唯一碳源的高效降解菌SL-1，经形态观察、生理生化分析以及16SrDNA基因序列分析，该菌株鉴定为假单胞菌（Pseudomonassp．）。28℃震荡培养10d后，菌株SL-1对5mg·L^-1苯并a]芘的降解率为35．7％；同时测定该降解菌的生长曲线、降解率变化曲线、最佳培养条件；土壤修复试验表明，42d时，降解菌SL-1对苯并芘的降解率达到76．3％。
|
| 关 键 词： | 苯并[a]芘 降解菌 筛选 鉴定 |
LSOLATION ,IDENTIFICATION AND DEGRADATION PROPERTIES OF A BENZO[a]PYRENE-DEGRADING STRAIN SL-1 |
| |
| LI Yanqiu,LIU Jun,GAO Peng,LIU Huijun,SUN Jianguang,ZHAO Ji,HU Haiyan,YI Li.LSOLATION ,IDENTIFICATION AND DEGRADATION PROPERTIES OF A BENZO[a]PYRENE-DEGRADING STRAIN SL-1[J].Inner Mongolia Environmental Protection,2010,22(2):74-77. |
| |
| Authors: | LI Yanqiu LIU Jun GAO Peng LIU Huijun SUN Jianguang ZHAO Ji HU Haiyan YI Li |
| |
| Institution: | 1 Inner Mongolia Academy of Coal Construction and Ecological Environment/a>;Hohhot 010020/a>;2 College of Life Science/a>;Inner Mongolia University/a>;Hohhot/a>;Hohhot 010018/a>;3 Institute of Agricultural Resources and Regional Planning/a>;Chinese Academy of Agriculture Science/a>;Beijing 100081 |
| |
| Abstract: | A bacterium strain SL-1 was isolated from soil by repeated subculture in liquid medium with benzoa]pyrene as sole carbon source.To confirm the classification of SL-1,morphological,physiological and biochemical characterization were applied and PCR was carried out to measure the gene sequence of 16S rDNA.The 16S rDNA was compared with related bacterias in GenBank,and phylogenetic tree was constructed.Based on the above results,the strain SL-1 was identified as Pseudomonas.sp..In a mineral salts medium with ... |
| |
| Keywords: | benzo[a]pyrene degrading strain screen identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
