长江南京段水体中有机污染物的遗传毒性研究

运用人外周血淋巴细胞彗星试验和蚕豆根尖细胞微核试验对长江南京段水体中有机污染物的遗传毒性进行了研究. 结果表明:长江南京段水体中的有机污染物对人外周血淋巴细胞和蚕豆根尖细胞均产生了不同程度的损伤,存在明显的遗传毒性,有机物是导致水体遗传毒性的主要因素. 试验结果与水体的有机污染状况基本一致. 彗星试验结果及趋势与微核试验相吻合,但前者更为敏感. 彗星试验和微核试验的结合使用在水环境的遗传毒性监测方面具有较大的应用价值. 

Evaluation of Genotoxic Effects Induced by Organic Pollutants in the Yangtze River in Nanjing Section

The comet assay of human peripheral blood lymphocytes and the micronucleus test of Vivia faba root tip cells were conducted to detect the genotoxic effects induced by the organics in the Yangtze River in Nanjing section. The results showed that DNA damages in lymphocytes and root tip cells were induced after exposure to organic extracts from water samples, river water possessed obvious genetic potentials and the organics was the main influence causing the genotoxicity. The experimental results could both reflect the pollution conditions of the local water body. The comet assay was more sensitive than the micronucleus test. The combination of the two tests has great value in monitoring the genotoxicity of water body.