转cry1Ab和epsps基因玉米C0030.3.5对大型蚤(Daphnia magna)的生态毒性研究

随着抗虫和耐草甘膦除草剂转基因玉米的迅速推广和种植,其环境安全性也越来越成为人们关注的焦点。为探讨抗虫耐除草剂转基因玉米C0030.3.5(外源基因cry1Ab和epsps)对水生动物环境的安全性,以模式生物大型蚤(Daphnia magna)为指示生物,分别使用1.5 g·L~(-1)C0030.3.5玉米粉和其非转基因对照DBN318玉米粉饲喂大型蚤28 d,探讨C0030.3.5玉米对大型蚤生长和繁殖的影响。结果显示,C0030.3.5玉米粉组大型蚤与亲本DBN318组大型蚤相比,体长、存活率、新生幼蚤总数等没有显著性差异(P0.05)。28 d饲喂实验结果表明,抗虫耐除草剂玉米C0030.3.5没有对大型蚤生长和繁殖产生不良影响。上述研究结果为转基因玉米的商业化种植和安全管理提供科学数据。

Ecotoxicological Effects of Genetically Modified Maize C0030.3.5 with cry1Ab and epsps Gene on Daphnia magna

Along with the rapid spread and plantation of maize that is resistant to insects and glyphosate, the environmental safety of genetically modified (GM) maize becomes the focus of attention. To discuss the environmental safety of genetically modified (GM) maize C0030.3.5 expressing the cry1Ab and EPSPS protein, water ?ea Daphnia magna, a crustacean arthropod commonly used as a model organism in ecotoxicological studies, were fed a basal diet of maize flours from C0030.3.5 or its parental maize DBN318 at the concentration of 1.5 g L^-1 (per individual per day) in a 28-day study. No significant differences were observed between D. magna feeding C0030.3.5 and non-GM flour on survival, body size and reproduction. These results suggested GM maize C0030.3.5 showed negative effect on neither growth nor reproduction of D. magna and provided a scientific basis for the commercial application and environmental safety management of the transgenic maize in China.