Effects of salinity on the clam <Emphasis Type="Italic">Chamelea gallina</Emphasis> haemocytes. Part II: Superoxide dismutase response |
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Authors: | Marta Monari Gian Paolo Serrazanetti Jurgen Foschi Valerio Matozzo Maria Gabriella Marin Otello Cattani |
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Institution: | (1) Department of Biochemistry “G. Moruzzi”, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia, Bologna, Italy;(2) Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy |
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Abstract: | The effects of different salinity levels (28, 34 and 40‰) on functional responses of Chamelea gallina haemocytes were evaluated in a two part study dealing with modulations of immune parameters. This part (Part II) of the study
was focused on the superoxide dismutase (SODs) activity and expression in haemocyte lysate and cell-free haemolymph. Results
of this study established that the exposure of C. gallina specimens at 40‰ salinity provoked a decrease in Mn-SOD and Cu/Zn-SOD activities in haemocyte lysate suggesting a declining
superoxide anion generation at the highest salinity tested. Expression of MnSOD was coherent with activity values, while Cu/ZnSOD
showed two immunoreactive bands. The former corresponds to the cytosolic Cu/Zn-SOD (16 kDa) was not coherent with the enzyme
activity and the second (28–30 kDa) probably attributed to EC-SOD. In cell-free haemolymph, Mn-SOD activity decrease and Cu/Zn-SOD
activity increase at 40‰ were observed, likely due to EC-SOD contribution, strongly induced at the same salinity. After EC-SOD
detection with two different antibody, we postulate that the EC-SOD like-protein band (29 kDa) may be constitute partly by
EC-SOD and probably by Cu/Zn-SOD dimeric form not completely dissociated under reducing condition or a Cu/Zn-SOD degenerated
but still recognized by antibody. The cell-free haemolymph increase of EC-SOD at high salinity values plays an important role
in immune defence of C. gallina. According to the conclusion of Part I of this study, our data confirmed the destabilizing effect of 40‰ salinity on haemocyte
functionality, while to 28‰ exposure, data don’t confirm its stressful action as instead stated by results of Part I. Further
studies are necessary to clear up this discrepancy. |
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