| 四膜虫彗星实验在环境水质检测中的应用 |
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| 引用本文： | 王丽,鲁志松,丁书茂,杨旭.四膜虫彗星实验在环境水质检测中的应用[J].环境科学与技术,2006,29(5):31-33. |
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| 作者姓名： | 王丽 鲁志松 丁书茂 杨旭 |
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| 作者单位： | 华中师范大学生命科学学院环境科学实验室,武汉,430079 |
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| 摘 要： | 介绍四膜虫作为生物材料在彗星实验水质检测中的应用,探讨了将单细胞原生生物应用于彗星实验在环境水质检测、遗传毒性检测等方面的应用优势和发展前景。研究结果表明:过氧化氢浓度与细胞损伤之间呈现良好的剂量-效应关系,东湖水和长江水未造成四膜虫DNA的损伤,印染厂污水对细胞有严重的损伤作用,可能会影响正常的生态环境。
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| 关 键 词： | 分子生物学 彗星实验 原生生物 四膜虫 水质检测 |
| 文章编号： | 1003-6504(2006)05-0031-03 |
| 收稿时间： | 2005-07-06 |
| 修稿时间： | 2005-08-31 |
Evaluation of Environmental Water Quality by Application of Comet Assay with Unicellular Protozoan Tetrahymena thermophila |
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| WANG Li,LU Zhi-song,DING Shu-mao,YANG Xu.Evaluation of Environmental Water Quality by Application of Comet Assay with Unicellular Protozoan Tetrahymena thermophila[J].Environmental Science and Technology,2006,29(5):31-33. |
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| Authors: | WANG Li LU Zhi-song DING Shu-mao YANG Xu |
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| Abstract: | Unicellular protozoan Tetrahymena thermophila was used in comet assay to detect DNA damage induced by wastewater so as to evaluate water quality. Results showed that a fine dosages-response relation between H2O2 and DNA damage was presented. Water from East Lake and the Yangtze River did not induce visible DNA damage while effluent from a printing and dyeing plant induced significant DNA damage which could impact on normal ecoenvironment. |
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| Keywords: | comet assay unicellular protozoan Tetrahymena thermophila: evaluation of water quality |
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