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Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay |
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| Authors: | Po-Min Kao Min-Che Tung Bing-Mu Hsu Yi-Chou Chiu Cheng-Yu She Shu-Min Shen Yu-Li Huang Wen-Chien Huang |
| Institution: | 1. Department of Earth and Environmental Sciences, National Chung Cheng University, Chiayi, Taiwan, Republic of China 2. Department of Surgery, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan, Republic of China 3. General Surgery, Surgical Department, Cheng Hsin General Hospital, Taipei, Taiwan, Republic of China 4. Department of Surgery, School of Medicine, National Yang Ming University, Taipei, Taiwan, Republic of China 5. Department of Safety Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, Kaohsiung, Taiwan, Republic of China 6. Division of Thoracic Surgery, Department of Surgery, Mackay Memorial Hospital, Mackay Medicine, Nursing, and Management College, Taipei, Taiwan, Republic of China |
| Abstract: | In this study, a SYBR green quantitative real-time PCR was developed to quantify and detect the Legionella spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and thermal spring area in Taiwan. Legionella was detected in 13.6 % (24/176), and the detection rate for river water, raw drinking water, and thermal spring water was 10, 21.4, and 16.6 %, respectively. Using real-time PCR, concentration of Legionella spp. in detected samples ranged between 9.75?×?104 and 3.47?×?105?cells/L in river water, 6.92?×?104 and 4.29?×?105?cells/L in raw drinking water, and 5.71?×?104 and 2.12?×?106?cells/L for thermal spring water samples. The identified species included Legionella pneumophila (20.8 %), Legionella jordanis (4.2 %), Legionella nautarum (4.2 %), Legionella sp. (4.2 %), and uncultured Legionella sp. (66.6 %). The presence of L. pneumophila in aquatic environments suggested a potential public health threat that must be further examined. |
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