Differences between 4-fluoroaniline degradation and autoinducer release by Acinetobacter sp. TW: implications for operating conditions in bacterial bioaugmentation

To develop a bacterial bioaugmentation system for fluorine-containing industrial wastewater treatment, optimal conditions for 4-fluoroaniline (4-FA) degradation and autoinducer release in Acinetobacter sp. TW were determined. Quorum sensing in biofilms of strain TW was also investigated. Different optimal conditions exist for 4-FA degradation and autoinducer release, particularly with regard to pH. Quorum sensing modulates extracellular polymeric substance (EPS) secretion and biofilm formation in the strain but plays no role in 4-FA degradation. Under optimal conditions for 4-FA degradation, the release of N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C₆-HSL) and N-hexanoyl-homoserine lactone (C₆-HSL) in strain TW was significantly lower than required for quorum sensing. Under optimal conditions for autoinducer release, on the other hand, 3-oxo-C₆-HSL and C₆-HSL levels exceeded the quorum sensing thresholds, thereby inducing EPS secretion and biofilm formation. We conclude that the optimal conditions for autoinducer release (25 °C, pH 5, 800 mg L^?1 4-FA, and 0 % NaCl) are suitable for bacterial colonization in bioaugmentation, while those for 4-FA degradation (25–30 °C, pH 8 and 800 mg L^?1 4-FA) maximize the system performance after colonization.