光照条件对跑道池光生物反应器内蛋白核小球藻生长特性的影响

本文采用多孔曝气管的曝气方式,以蠕动泵为藻液循环的动力,研究了气体流量、光照强度、光照周期等因素对跑道池光生物反应器内蛋白核小球藻生长与代谢特性的影响,并利用傅里叶红外光谱半定量法对不同光照条件下藻细胞内蛋白质、油脂以及多糖含量变化进行了分析。研究结果表明,适当的气体流量、光照强度和光照周期有利于跑道池内蛋白核小球藻细胞的生长和代谢。在本实验条件下,在最佳气体流量2 000 mL/min条件下,光照强度为3 500 μW/cm2,光照周期为16:8时最有利于蛋白核小球藻的生长;光照强度为2 000 μW/cm2和光照周期为16:8的条件均有利于藻细胞内油脂的积累。

Effect of light conditions on growth of Chlorella pyrenoidosa in an open raceway photobioreactor

In this work, the effects of gas flow rate, light intensity and photoperiod on characteristics of growth and metabolism of Cholorella pyrenoidosa were investigated in an open raceway photobioreactor fixed aeration pipe with many holes for aeration, and the culture solution was cycled by peristaltic pump. The contents of protein, lipid and polysaccharide of the microalgae cells from different light conditions were analyzed through semi-quantitative method by using Fourier transform infrared spectroscopy. The research results indicate that optimal gas flow rate, illumination intensity and photoperiod could facilitate the growth and metabolism of Chlorella pyrenoidosa. In the experiment, the optimal growth performance of algae cells were achieved at a gas flow rate 2 000 mL/min and a light intensity of 3 500 μW/cm2 with a photoperiod of 16:8, while the maximal lipid accumulation could be obtained at a light intensity of 2 000 μW/cm2.