儿茶酚抑制藻细胞活性过程中IOM的三维荧光特性

以天然水体中藻类为研究对象,研究了儿茶酚对藻类的抑制效应,并采用三维荧光法分析了抑藻过程中胞内有机物的释放及降解情况。水样A和水样B分别采自某护城河和一景观水体。结果显示,儿茶酚能够有效抑制实际水体中藻细胞活性,当儿茶酚终浓度为10 mg/L培养6 d后,水样A和水样B的抑藻率分别达90%和94%,且抑藻率随着投加浓度的增加、培养时间的延长相应增加。在投加儿茶酚后,水样类蛋白荧光峰强度显著增加,但随着培养时间的延长,峰强均有所降低,细胞释放的类蛋白物质发生降解。当投加儿茶酚浓度低于1 mg/L时,藻细胞活性受轻度抑制,会释放类富里酸物质,当儿茶酚投加浓度高于5 mg/L,藻细胞活性受到强烈抑制,不会释放类富里酸物质。投加儿茶酚会增大水样COD浓度,但对水样TN浓度没有影响。

Fluorescence Characterization of IOM from Inhibitory Effect on Algal Activity by CAT

Inhibitory effect of catechol(CAT)on algal activity from natural waters as well as the release and degradation of intracellular organic matters(IOM)resulting from algal inhibition by three-dimensional fluorescence spectroscopy were studied.The algae A and B were sampled from a city moat and a landscape water respectively.Results showed that CAT has significant inhibitory effect on activity of algae,which could reach 90% for sample A and 94% for sample B after 6d at final concentration of CAT 10 mg/L.During the culture period,inhibition ratio increased along with CAT concentration and culture time.Just after adding of CAT,the protein-like fluorescence peak intensity of algal samples increased significantly,but gradually declined over the culture time.Protein-like fluorescent matters released from algal cells could be easily degraded.When concentration of CAT in water was less than 1 mg/L,algal activity was slightly inhibited and then algal cells released fulvic-like substances.While the CAT was greater than 5 mg/L,it caused strongly inhibition effect,and algal cells would not release fulvic-like substances.Addition of CAT would result in the increase of COD concentration,but the concentration of TN was not affected.