高盐废水MFCs不同阴极电子受体产电及微生物群落分析

为探查不同电子受体产电性能及对阳极微生物群落的影响，研究了3种电子受体(铁氰化钾、曝气阴极、过硫酸钾)，构建了双室榨菜废水微生物燃料电池系统(microbial fuel cells，MFCs)，实现了污水处理和能量回收的双重目的，探讨了不同电子受体(铁氰化钾、曝气阴极、过硫酸钾)对榨菜废水MFCs产电性能及阳极微生物群落的影响。结果表明：在产电性能方面，当过硫酸钾作为阴极电子受体时，电池输出电压、库仑效率、功率密度均优于另外2种常用阴极电子受体(铁氰化钾和氧气)；在500 Ω的外接电阻间歇运行的条件下，其输出电压、库仑效率、功率密度分别为802 mV、(33±1.6)%、697 mW·m⁻²。阳极生物16S rRNA基因测序分析表明，水解发酵菌为榨菜废水微生物燃料电池阳极核心菌群，铁氰化钾、氧气和过硫酸钾MFCs阳极微生物菌群相对丰度分别为64.3%、63.6%和75.51%，包括Lentimicrobium、Synergistaceae、Sphaerochaeta、Anaerolineaceae、Draconibacteriacea菌属。阴极电子受体不同的MFCs的阳极微生物群落核心菌群类似，但是丰度有所不同。势差较大的电子受体(过硫酸钾)微生物群落多样性和丰富度较高，产电和污染物去除效果较好。

Electricity generation and microbial community analysis of different cathodic electron acceptors in MFC treating mustard tuber wastewater

In order to explore the relationship between the electrical properties of different electron acceptors and the anodic microbial community, three electron acceptors (potassium ferricyanide, aerated cathode, potassium persulfate) were experimentally studied. The microbial fuel cells (MFCs) of double-chamber mustard wastewater were constructed to achieve the dual purpose of sewage treatment and energy recovery. Effects of different electron acceptors (potassium ferricyanide, aerated cathode and potassium persulfate) on their electricity production performance and anode microbial community in mustard tuber wastewater were investigated. Results show that when potassium persulfate was used as the cathode electron acceptor, electricity production performance, such as the battery output voltage, coulombic efficiency and power density, was better than the other two common cathode electron acceptors (potassium ferricyanide and oxygen). Under the intermittent operation conditions with 500 Ω external resistors, the output voltage, coulombic efficiency, and power density were 802 mV, (33±1.6)% and 697 mW·m−2, respectively. The 16S rRNA gene sequencing analysis of the anode organism showed that the hydrolysis fermentation bacteria were the anode core bacteria of MFC treating mustard tuber wastewater, which accounted for 64.3%, 63.6%and 75.51% in the microbial flora of potassium ferricyanide, oxygen and potassium persulfate MFCs, respectively, and included Lentimicrobium, Synergistaceae, Sphaerochaeta, Anaerolineaceae, Draconibacteriacea. MFCs with different cathode electron acceptors had similar core bacteria of anode microflora, but had different abundance. The electron acceptor (potassium persulfate) with large potential difference caused higher diversity and richness of microbial community, and had better performance on electricity generation and pollutant removal than other electron acceptors.