丛枝菌根真菌对柑橘组培苗生长和抗氧化酶的影响

在温室条件下以枳〔Poncirus trifoliata(L.)Raf.〕组培苗为试材,研究了接种Glomus versiforme和G.mosseae对其生长、碳水化合物和抗氧化酶的影响.结果表明,接种G.versiforme的组培苗和接种G.mosseae的组培苗分别在第二级侧根和第一级侧根中观察到最高的菌根侵染率、泡囊数、丛枝数和侵入点.两种丛枝菌根真菌都显著提高了茎粗、叶面积、叶片数、根系体积、地上部干重、地下部干重、叶绿素和类胡萝卜素含量.两种丛枝菌根真菌显著促进了叶片和根系可溶性糖以及总的非结构碳水化合物含量.丛枝菌根真菌也提高了叶片和根系中SOD、POD和CAT活性,但显著抑制了叶片和根系中可溶性蛋白含量.G.versiforme对柑橘组培苗生长和碳水化合物的促进效果较好;G.mosseae对组培苗抗氧化酶的促进效果较好.表4参26

Effects of Arbuscular Mycorrhizal Fungi on the Growth and Antioxidant Enzymes of Micropropagated Citrus

Inoculation effects with the arbuscular mycorrhizal (AM) fungi, Glomus versiforme (Karsten) Berch and G. mosseae ( Nicol. & Gerd. ) Gerdemann & Trappe, on the growth, carbohydrate and antioxidant enzymes were analyzed in micropropagated trifoliate orange (Poncirus trifoliata (L.) Raf. ) in pots under greenhouse conditions. After 300 days' inoculation, the root colonization and the numbers of arbuscules, vesicles and entry points were the highest in the 2nd lateral roots inoculated with G. versiforme and in the 1 st lateral roots colonized by G. mosseae. Stem diameter, leaf area, leaf number per plant, root volume, shoot and root dry weights, chlorophyll and carotenoid contents markedly increased under inoculated conditions. Inoculated plants showed the greater soluble sugar and total non-structural carbohydrate (NSC) contents in leaves and roots, and superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were higher in mycorrhizal plants than those in non-mycorrizal plants. However, a severe depression was found in the soluble protein contents of mycorrhizal leaves and roots. G. versiforme was more effective than G. mosseae in growth and carbohydrate increments, while G. mosseae was more effective than G. versiforme in antioxidant enzymes increments. Tab 4, Ref 26