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| 城市污水处理厂活性污泥中氨氧化菌群落结构研究 | |
| 引用本文： | 王晓慧,文湘华,Criddle Craig,张杰,杨宁宁,赵颖.城市污水处理厂活性污泥中氨氧化菌群落结构研究[J].环境科学,2009,30(10):3002-3006. |
| 作者姓名： | 王晓慧 文湘华 Criddle Craig 张杰 杨宁宁 赵颖 |
| 作者单位： | 1. 清华大学环境科学与工程系,北京,100084 2. Department,of,Civil,and,Environmental,Engineering,Stanford,University,Stanford,CA94305-4020,USA 3. 北京排水集团,北京,100044 |
| 基金项目： | 国家科技支撑计划项目(2006BAC19B01-02) |
| 摘 要： | 为了解析污水处理厂中氨氧化菌(AOB)的群落结构,以及处理工艺、规模等参数对AOB群落结构的影响,采用针对AOB功能基因氨单加氧酶(amoA)的末端限制性片段长度多态性技术(T-RFLP)、克隆测序等方法,研究了北京市6个污水处理厂的9个污水处理系统中AOB的群落结构.T-RFLP指纹图谱表明不同污水处理系统中AOB的群落结构有所不同,主要的末端限制性片段(T-RF)为354、491和291 bp.T-RFLP指纹图谱及聚类分析表明,污水处理工艺对系统中AOB的群落结构影响较小,而处理规模对AOB的群落结构有一定的影响.对功能基因amoA的系统发育分析表明,污水处理系统中优势AOB均为Nitrosomonasspp.,而非Nitrosospiraspp..这可能是Nitrosomonasspp.的最大比增长速率(μmax)较高,使其更容易成为活性污泥系统中的优势AOB. |
| 关 键 词： | 氨氧化菌（AOB） 末端限制性片段长度多态性技术（T-RFLP） 氨单加氧酶（amoA） 城市污水处理厂 |
| 收稿时间： | 2008/11/11 0:00:00 |
| 修稿时间： | 3/4/2009 12:00:00 AM |
Community Structures of Ammonia-oxidizing Bacteria in Activated Sludge of Wastewater Treatment Plants |
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| WANG Xiao-hui,WEN Xiang-hu,Criddle Craig,ZHANG Jie,YANG Ning-ning and ZHAO Yin.Community Structures of Ammonia-oxidizing Bacteria in Activated Sludge of Wastewater Treatment Plants[J].Chinese Journal of Environmental Science,2009,30(10):3002-3006. | |
| Authors: | WANG Xiao-hui WEN Xiang-hu Criddle Craig ZHANG Jie YANG Ning-ning and ZHAO Yin |
| Institution: | 1.Department of Environmental Science and Engineering;Tsinghua University;Beijing 100084;China;2.Department of Civil and Environmental Engineering;Stanford University;Stanford CA94305-4020;USA;3.Beijing Drainage Group Co.Ltd.;Beijing 100044;China |
| Abstract: | In order to investigate the community structure of ammonia oxidizing bacteria(AOB) in wastewater treatment plants and the influence of treatment process and system scale on AOB community.We investigated AOB communities in activated sludges collected from 9 wastewater treatment systems,using specific PCR followed by terminal restriction fragment length polymorphism(T-RFLP),cloning,and sequencing of amoA genes.The T-RFLP fingerprints analysis shows that there are some differences among the AOB community struc... |
| Keywords: | ammonia-oxidizing bacteria (AOB) terminal restriction fragment length polymorphism (T-RFLP) amoA wastewater treatment plant |
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