离子液体氯化1-辛基-3-甲基咪唑对酵母细胞增殖生长和细胞膜通透性的影响

为探讨咪唑类离子液体氯化1-辛基-3-甲基咪唑([C₈mim][Cl])对酵母细胞的增殖生长和细胞膜通透性的影响，以不同浓度的[C₈mim][Cl]处理酵母细胞，研究离子液体对酵母细胞增殖和菌落形成的影响，通过测定酵母细胞外液蛋白质和核酸的含量，判断膜通透性的大小和[C₈mim][Cl]对细胞膜性结构的损伤。结果显示，0.1 mmol·L^-1的[C₈mim][Cl]延长了酵母细胞到达对数生长期的时间，在6～9 h之间对酵母细胞的增殖存在明显的抑制作用。1 mmol·L^-1的[C₈mim][Cl]使酵母细胞增殖不能到达对数生长期，对酵母细胞的增殖一直具有较强的抑制作用。随着离子液体浓度的增加，小菌落的数量增多。当平板内[C₈mim][Cl]浓度达到10 mmol·L^-1时，完全抑制了菌落的形成。[C₈mim][Cl]处理酵母细胞后，细胞外液中OD₂₈₀、OD₂₆₀的值显著升高。研究表明，细胞膜等膜性结构通透性的增加是离子液体[C₈mim][Cl]抑制酵母细胞增殖生长的原因之一。

Effect of 1-Octyl-3-Methylimidazolium Chloride on the Growth, Cell Proliferation and Membrane Permeability in Yeast Saccharomyces cerevisiae

To study the effect of 1-octyl-3-methylimidazolium chloride on the growth, cell proliferation and membrane permeability in yeast Saccharomyces cerevisiae, yeast cells were exposed to [C₈mim][Cl] with different concentrations. The proliferation of yeast cells was examined and the number and size of yeast colonies were evaluated in this study. Membrane permeability and its damage by [C₈mim][Cl] were also detected by measuring OD₂₈₀ and OD₂₆₀ of extracellular fluid. The results showed that the treatment of yeast cells with 0.1 mmol·L^-1 [C₈mim][Cl] extended the time to reach the logarithmic phase time, and significantly inhibited cell proliferation between 6 h and 9 h. The yeast cell proliferation was inhibited and was unable to reach the logarithmic growth phase when treated with 1 mmol·L^-1[C₈mim][Cl]. With increasing the concentration of [C₈mim][Cl], the number of small colonies increased. When the concentration of [C₈mim][Cl] reached 10 mmol·L^-1, no colony was formed in the plate medium. The values of OD₂₈₀ and OD₂₆₀ of extracellular fluid of yeast cells increased significantly after treated with [C₈mim][Cl]. The results suggested that [C₈mim][Cl] inhibited the growth and proliferation of yeast cells partially through increasing cell membrane permeability.