| 环境因素对大肠杆菌REP-PCR指纹图谱稳定性影响研究 |
| |
| 引用本文： | 韩彦垒,梁斌,李冬梅,苏洁,关道明,王耀兵.环境因素对大肠杆菌REP-PCR指纹图谱稳定性影响研究[J].海洋环境科学,2011,30(5):614-617. |
| |
| 作者姓名： | 韩彦垒 梁斌 李冬梅 苏洁 关道明 王耀兵 |
| |
| 作者单位： | 1. 国家海洋环境监测中心,辽宁大连116023;大连海洋大学,生命科学与技术学院,辽宁大连116023
2. 国家海洋环境监测中心,辽宁大连,116023 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)“典型近岸海域粪便污染源的MST监测技术研究”(2007AA09Z111) |
| |
| 摘 要： | 大肠杆菌作为粪便污染指示生物,其DNA指纹图谱稳定性是制约微生物污染源识别技术发展的瓶颈之一.本研究采用绿色荧光蛋白基因(GFP)标记后的Escherichia coli K12(K12GFP)为受试菌株,利用REP-PCR方法,对大肠杆菌在不同温度、光照、盐度条件下其DNA指纹图谱的稳定性进行了研究.结果表明,绿色荧...
|
| 关 键 词： | 大肠杆菌 DNA指纹图谱稳定性 REP-PCR 绿色荧光蛋白 |
Effect of environmental factors of REP-PCR fingerprinting patterns stability of Escherichia coli |
| |
| HAN Yan-lei,LIANG Bin,LI Dong-mei,SU Jie,GUAN Dao-ming,WANG Yao-bing.Effect of environmental factors of REP-PCR fingerprinting patterns stability of Escherichia coli[J].Marine Environmental Science,2011,30(5):614-617. |
| |
| Authors: | HAN Yan-lei LIANG Bin LI Dong-mei SU Jie GUAN Dao-ming WANG Yao-bing |
| |
| Institution: | HAN Yan-lei1,2,LIANG Bin1,LI Dong-mei1,SU Jie1,GUAN Dao-ming1,WANG Yao-bing1(1.National Marine Environmental Monitoring Center,Dalian 116023,China,2.College of Life Science and Technology,Dalian Ocean University,China) |
| |
| Abstract: | Escherichia coli is an important indicator in fecal pollution,The E.coli DNA fingerprinting stability is to restrict one of identifying techniques development of the microbial polluted sources.In order to solve this problem,the E.coli K12(K12 GFP),which the green fluorescent protein(GFP) plasmid was transformed,were chose to make a research.Its DNA fingerprinting stability was under different environmental factors(temperature,sunlight time and salinity).The results showed that the green fluorescent protein(... |
| |
| Keywords: | Escherichia coli DNA fingerprinting stability REP-PCR green fluorescent protein |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
