软脂酸和硬脂酸对铜绿微囊藻生长的抑制作用

研究了软脂酸和硬脂酸对产毒铜绿微囊藻Microcystis aeruginosa生长的抑制效应,比较了长链饱和脂肪酸抑藻的构效关系。选用的藻种是购于中国科学院水生生物研究所的产毒铜绿微囊藻Microcystis aeruginosa FACHB-912,且实验藻种处于对数生长期。软脂酸和硬脂酸在藻液中的4种最终质量浓度为：30、60、90和120mg·L-1。实验周期为6d,每天定时定量取藻液,测量A（650nm）,叶绿素a含量,藻蓝蛋白含量。实验结束后,采用SPSS（13.0版本）软件和DPS（7.05版本）分析加入这2种脂肪酸的藻液的3个指标与空白对照之间的差异显著性。结果表明,2种饱和脂肪酸的4种质量浓度对产毒铜绿微囊藻均有显著的抑制作用,浓度越大,抑藻效果越明显。其中,2种饱和脂肪酸在相同浓度的条件下,软脂酸比硬脂酸的抑制作用要明显。实验技术方面有2个创新点：①因为长链饱和脂肪酸无法溶于蒸馏水,实验采用乙醇来溶解脂肪酸。②因为溶于乙醇的长链饱和脂肪酸溶液无法用高压灭菌锅灭菌,实验采用微孔滤膜对脂肪酸溶液进行灭菌。

The inhibitory effects of palmitic acid and stearic acid on Microcystis aeruginosa

The inhibitory effects of palmitic acid and stearic acid on toxic Microcystis aeruginosa were studied, and the relationship between the structures of the long-chain saturated fatty acids and the inhibitory effects was compared. Our experiment selected toxic Microcystis aeruginosa FACHB-912, which was bought from Institute Hydrobiology of Chinese Academy of Sciences, and made it in the logarithmic phase. The four concentrations of palmitic acid and stearic acid in the algal solutions were: 30 mg·L~(-1), 60 mg·L~(-1), 90 mg·L~(-1) and 120 mg·L~(-1). The experimental time was six days, and we measured A650nm, chlorophyll a and phycocyanin of the alga every day. When the experiment was finished, we used SPSS (13.0 Version) and DPS (7.05 Version) to analyze the significant differences between the alga treated by the fatty acids and the control. The result showed that the four concentrations of the two saturated fatty acids could all inhibit the growth of the alga obviously. The higher the concentration, the obvious the inhibitory effect was found. Also, under the same concentration, the inhibitory effect of palmitic acid on the alga was more obvious than that of stearic acid. Our experiment also had two innovations: ①Because long-chain saturated fatty acids cannot be dissolved in water, we used ethanol to dissolve them. ②Because ethanol solution of long-chain saturated fatty acids cannot be sterilized by autoclaves, we used microfiltration membranes to sterilize the fatty acid solutions.