| 微囊藻中叶绿素a提取方法的优化 |
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| 引用本文： | 李胜生,董元华,刘云,张劲强.微囊藻中叶绿素a提取方法的优化[J].环境监测管理与技术,2008,20(4):43-45. |
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| 作者姓名： | 李胜生 董元华 刘云 张劲强 |
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| 作者单位： | 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏,南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏,南京,210008;中国科学院研究生院,北京,100049
2. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,江苏,南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,江苏,南京,210008 |
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| 基金项目： | 国家科技支撑计划基金，中国科学院创新性基金 |
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| 摘 要： | 用组织研磨、浸泡提取、热浴超声3种提取方法和丙酮、甲醇、乙醇3种提取溶剂，从实验室纯培养微囊藻中提取叶绿素a，并以高效液相色谱法测定其质量浓度。结果表明，经组织研磨破碎藻细胞后用甲醇提取的效果好于丙酮和乙醇，经浸泡和超声破碎藻细胞后用乙醇的提取效果好于丙酮和甲醇；使用乙醇-热浴超声法在所有的试验组合中获得最佳提取效果，条件为：乙醇温度为50℃-55℃，超声6min-8min后再静置提取5h-6h。
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| 关 键 词： | 微囊藻 叶绿素A 提取方法 |
The Optim ization of Extractive M ethodsfor Chlorophyll 2 a from M icrocystis |
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| LI Sheng-sheng,DONG Yuan-hua,LIU Yun,ZHANG Jin-qiang.The Optim ization of Extractive M ethodsfor Chlorophyll 2 a from M icrocystis[J].The Administration and Technique of Environmental Monitoring,2008,20(4):43-45. |
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| Authors: | LI Sheng-sheng DONG Yuan-hua LIU Yun ZHANG Jin-qiang |
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| Institution: | LI Sheng-sheng , DONG Yuan-hua , LIU Yun , ZHANG Jin-qiang ( 1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences,Nanjing, Jiangsu 210008, China; 2. Soil and Environment Joint Open Laboratory between Institute of Soll Science, Chinese Academy of Sciences and Hong Kong Baptist University, Nanjing, Jiangsu 210008, China ; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China) |
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| Abstract: | The methods （tissue grinding, immersion, ultrasonic thermal bath） and solvents （acetone, methanol, ethanol） were tasted for chlorophyll-a extraction from microcystis. The results indicated that extraction effect of chlorophyll a by method of tissue grinding to break algae ceils with methanol was higher than that with acetone or ethanol, and that by method of immersion or ultrasonic thermal bath with ethanol was better than with acetone or methanol. In all test groups, the best way was obtained by 6 min - 8 min ultrasonic treatment and then 5 h -6 h static extraction in the 50 ℃ -55℃ethanol thermal bath. |
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| Keywords: | M icrocystis Chlorophyll-a Extraction method |
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