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渤海排污口邻近海域异养细菌的组成与分布
引用本文:刘霜,李永霞,刘旭东,王亚楠,孙蓓蓓,宋帅.渤海排污口邻近海域异养细菌的组成与分布[J].环境监测管理与技术,2014,26(1):22-25.
作者姓名:刘霜  李永霞  刘旭东  王亚楠  孙蓓蓓  宋帅
作者单位:国家海洋局海洋溢油鉴别与损害评估技术重点实验室 国家海洋局北海环境监测中心;黄海水产研究所鳌山卫基地;国家海洋局海洋溢油鉴别与损害评估技术重点实验室 国家海洋局北海环境监测中心;青岛市海洋与渔业局;国家海洋局海洋溢油鉴别与损害评估技术重点实验室 国家海洋局北海环境监测中心;国家海洋局海洋溢油鉴别与损害评估技术重点实验室 国家海洋局北海环境监测中心
基金项目:基金项目:〖HTSS〗海洋公益性行业科研专项基金资助项目(201105007)
摘    要:对渤海4个重点监测排污口(北塘、大蒲河、弥河和虞河)邻近海域异养细菌的组成和分布进行了调查研究。结果表明,分离到的各排污口邻近海域的细菌隶属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Acti-nobacteria)和拟杆菌门(Bacteroidetes)4个细菌类群,其中变形菌门的细菌最丰富。各排污口的优势菌有所不同,北塘、弥河2个采样点的优势菌分别为Bacillus sp.、Pseudoalteromonas sp.,大蒲河采样点的优势菌为Vibrio sp.和Oceanospirillum sp.,虞河采样点的优势菌为 Shewanella sp.和 Bacillus sp.。多维尺度分析表明,北塘和虞河采样点的细菌种群特征较相近,细菌种群分布特征可能与排污口的海洋环境有一定的相关性。

关 键 词:排污口  海洋异养细菌  16SrDNA  优势菌  多维尺度分析  渤海

Diversity and Distribution of Marine Heterotrophic Bacteria Influenced by Discharge Outlets in Bohai Area
LIU Shuang,LI Yong-xia,LIU Xu-dong,WANG Ya-nan,SUN Bei-bei,SONG Shuai.Diversity and Distribution of Marine Heterotrophic Bacteria Influenced by Discharge Outlets in Bohai Area[J].The Administration and Technique of Environmental Monitoring,2014,26(1):22-25.
Authors:LIU Shuang  LI Yong-xia  LIU Xu-dong  WANG Ya-nan  SUN Bei-bei  SONG Shuai
Institution:1. Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology ,SOA , Oingdao , Shandong 266033, China ; 2. North China Sea Environment Monitoring Center,SOA, Oingdao,Shandong 266033, China; 3. The Base in Aoshanwei of Yellow Sea Fisheries Research Institute, Oingdao ,Shandong 266237, China ; 4. Ocean and Fisheries Bureau of Oingdao, Oingdao,Shandong 266071, China)
Abstract:An investigation was done that the diversity and distribution of heterotrophic bacteria in the adja-cent area of four main monitored discharge outlets in Bohai,which located in Beitang,Dapuhe,Mihe and Yuhe,re-sectively.The results showed that all the isolated bacterial strains belonged to four phyla,including Proteobacteria, Firmicutes,Actinobacteria and Bacteroidetes,in which Proteobacteria was the dominant phyla.Furthermore,the dominant bacteria of four sampling locations are different.To be specific,Bacillus sp.and Pseudoalteromonas sp. were found to be dominant in Beitang and Mihe,respectively.Vibrio sp.and Oceanospirillum sp.were prevailing in Dapuhe,while the dominant bacteria of Yuhe were Shewanella sp.and Bacillus sp.Results of MDS analysis indica-ted that the bacterial population characteristics from Beitang and Yuhe were similar to each other,suggesting that the diversity and distribution of bacteria probably be related with the marine environment.
Keywords:Discharge outlet  Marine heterotrophic bacteria  16S rDNA  Dominant strain  MDS  Bohai
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