| DAPI染色直接定量测定细菌胞内多聚磷酸盐 |
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| 引用本文： | 王鑫,张文,陈旭,朱雯,杨柳燕.DAPI染色直接定量测定细菌胞内多聚磷酸盐[J].环境监测管理与技术,2015,27(6):40-43. |
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| 作者姓名： | 王鑫 张文 陈旭 朱雯 杨柳燕 |
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| 作者单位： | 四川省辐射环境管理监测中心站;四川省辐射环境管理监测中心站;中国科学院高能物理研究所 |
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| 基金项目： | 国家水体污染控制与治理重大专项基金资助项目（2012ZX07101006）； 江苏省科技厅课题基金资助项目（BE2012736） |
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| 摘 要： | 采用DAPI将细菌胞内或活性污泥中多聚磷酸盐(Polyp)染色,用酶标仪测定DAPI-Polyp复合物发射荧光的强度,以此定量细菌胞内的Polyp。用该方法和镜检法同时测定异染粒含量差异显著的菌株胞内Polyp,2种方法测定结果一致。异染粒含量差异不显著的菌株胞内Polyp定量分析表明,该方法灵敏度高于镜检法和废水总磷间接测定法。
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| 关 键 词： | 总α 总β 饮用水水源地 地震灾区 芦山 |
Direct Quantitative Determination Method of Bacterial Polyphosphate |
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| Abstract: | In order to investigate the content of polyphosphate (Polyp) in bacteria and active sludge,a direct quantitative method using DAPI dyeing combined with fluorescence detection in a microplate reader was probed. When bacteria strains possessing significant different levels of intracellular polyp were used as the testing samples,identical results were obtained by using this method and traditional microscopic observation. Further quantitative analysis with bacteria samples possessing similar amount of intracellular polyp showed that this measuring method had higher sensitivity compared with other methods,microscopic observation,total phosphate analysis in the bulk liquid,etc. |
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