| 用Ames试验检测水源水和自来水中的遗传毒性 |
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| 引用本文： | 方 东,梅卓华,楼 霄,章 弼.用Ames试验检测水源水和自来水中的遗传毒性[J].环境监测管理与技术,2000,12(1):20-22. |
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| 作者姓名： | 方 东 梅卓华 楼 霄 章 弼 |
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| 作者单位： | 南京市环境监测中心站!江苏南京210013 |
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| 摘 要： | 用微伤寒沙门氏菌／哺乳动物微粒体酶系的Ａｍａｅｓ试验，研究了不同季节物水源水及管网自来水中的遗传毒性，以ＸＡＤ２树脂为吸附剂，以丙酮－甲醇的混合液为洗脱液，浓率水样中的有机物，并对部分阳性水样进行有机成分的定性分析。结果发现：１３个水样中有７个样品在淡需要代谢活化系统Ｓ９的情况下，可诱导鼠伤寒沙门氏菌碱基移码型菌株的回复突变；不同水样在不同季节不同的诱导作用；同时通过ＧＣ／ＭＳ方法分析，发现阳性水
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| 关 键 词： | Ames试验 GC＼MS 水源水 自来水 遗传毒性 |
Determination of Genetic Toxicity of Source Water and Drinking Water by AMES Test |
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| FANG Dong,MEI Zhuo hua,LOU Xiao,ZHANG Bi.Determination of Genetic Toxicity of Source Water and Drinking Water by AMES Test[J].The Administration and Technique of Environmental Monitoring,2000,12(1):20-22. |
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| Authors: | FANG Dong MEI Zhuo hua LOU Xiao ZHANG Bi |
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| Abstract: | Genetic toxicity of source water and drinking water in different seasons was researched by Ames test with the application of Salmonella/microsonal enzyme of mammal.After the adsorption of organics with XAD 2 as adsorbant and acetone methanol as desorbant,organics in positive samples were identified.The results showed that 7 of 13 samples led reversion mutation of base shift mutagenisis without metabolic activation S 9, and different samples from different seasons had different leading function. GC/MS analysis showed that positive samples had pollutants in US EPA 129 priority pollutants or other lists of priority pollutants, which meant the water was polluted. |
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| Keywords: | Ames test GC/MS Source water Drinking water Genetic toxicity |
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