M12半自动净化法与手工净化法应用于飞灰中二噁英类检测比较

研究比较了M12半自动净化法与手工净化法应用于二噁英检测的效果。结果显示,采用高分辨气相色谱-高分辨质谱法(HRGC-HRMS)检测飞灰样品中的二噁英,手工净化法的回收率要优于M12半自动净化法的回收率,但M12半自动净化法除2种单体外,其余单体回收率均满足对二噁英的分析要求,且2种净化方法所得二噁英的总毒性当量结果相近,证明手工净化法与HRGC-HRMS联用检测飞灰中二噁英的效果较好,但M12半自动净化法也能够满足HRGC-HRMS检测二噁英的净化要求。应用酶联免疫法检测飞灰样品中二噁英的总毒性量,当样品中二噁英浓度较高时,M12半自动净化法和手工净化法净化测得结果与基准值一致性较好;当样品中二噁英浓度较低时,手工净化法检测结果与基准值差别较大,表明酶联免疫法更适合用M12半自动净化法进行净化。

Comparison of Dioxin Analysis in Fly Ash by the M12 Semi Automatic Cleanup Method and Manual Cleanup Method

The effect of using the M12 semi-automatic cleanup method and manual cleanup method for the determination of dioxins was studied and compared .The results showed that the recovery rates obtained using the manual method were better than the M 12 semi-automatic cleanup method in high resolution gas chromatography -mass spectrometry (HRGC -HRMS) for the determination of dioxin in fly ash.Except for two types of monomer ,the recovery rates of rest monomers obtained using the M 12 semi-automatic puri-fication method were all qualified for the analysis requirement of dioxins ,and the results of the total toxic equivalent were similar u -sing the two methods.The results indicated that combining the manual cleanup method with HRGC -HRMS was better than that of the M12 semi-automatic purification method,although the latter could also meet the requirement .The total toxicity of dioxins in fly ash samples was analyzed by enzyme linked immunosorbent assay (ELISA).The results were in good agreement with the reference values using both of the cleanup methods when the concentrations of dioxins were high in the sample .When the concentrations of dioxins in the sample were low,the results obtained using the manual method were more deviated from the reference values ,which in-dicates that the M12 semi-automatic purification method was more suitable for the ELISA method .