浮游动物DNA宏条形码多样性监测采样方法研究

浮游动物是水生生态系统的重要组成部分,对环境因子敏感,是水生生态健康状况的重要指示生物.基于传统形态学的浮游动物调查方法无法鉴定桡足类幼体,难以准确了解浮游动物群落组成.DNA宏条形码技术基于DNA序列差异识别物种,为浮游动物群落组成的准确解析提供了新方法.比较了不同浮游动物采样方法(直接过滤法和网富集法)对浮游动物D...

Study on Methods of Zooplankton Sampling for DNA Metabarcoding Monitoring

Zooplankton is an important part of aquatic ecosystem,which is sensitive to environmental factors and indicates the health status of aquatic ecology.Traditional morphological zooplankton survey can not identify copepod nauplius,and it is difficult to accurately understand zooplankton community composition.DNA macrobarcoding provides a new method for the accurate analysis of zooplankton community composition based on DNA sequence differences.In this study,the effects of different sampling methods(water filtration method and net enrichment method)and sampling volume on zooplankton biodiversity monitoring were compared.The results showed that different sizes of zooplankton should adopt different sampling methods,among which direct filtration of 1 L water sample is the best for rotifer diversity,and enrichment of 5~10 L water sample with zooplankton net is more suitable for monitoring cladocerans and copepods.This study initially achieved the standardization of zooplankton metabarcoding monitoring sampling,and also provided technical support for the future aquatic biodiversity monitoring by metabarcoding technology.