DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物

建立了DNPH衍生化采样-溶剂解析-高效液相色谱测定木制品中醛酮化合物的方法。采样时需在2,4-二硝基苯肼吸附管前串联一只SEP-PAK脱臭氧小柱;采样体积应≤10 L,流量≤400 m L/min。该法对25种醛(酮)化合物对应的衍生物分离效果良好,在0.005~3 mg/L范围内线性良好,相关系数R2≥0.999 6,回收率为85.3%~113.5%,精密度为2.67%~4.56%。采样量为10 L时,25种醛(酮)化合物的检出限和定量限分别≤0.16和0.50μg/m3。

Simultaneous Determination of 25 Aldehydes and Ketones in Wood Materials by 2,4-Dini-trophenylhydrazine (DNPH) Derivatization Sampling and Solvent Desorption Coupled with High Performance Liquid Chromatography

The measurement of aldehydes and ketones in wood materials was established using 2,4-dinitrophenylhydrazine (DNPH) derivatization sampling and solvent desorption coupled with high performance liquid chromatography ( HPLC) .A SEP-PAK column was attached to the front of the DNPH sampling column .Sample volume and flow speed were maintained below 10 L and 400 mL/min, re-spectively.The method showed good separation between 25 aldehydes and ketones , with good linearity in the range of 0.005 ~3 mg/L.The correlation coefficient R2 was above 0.999 6, and the recovery rates and precision were in the range of 85.3%~113.5%and 2.67%~4.56%, respectively.When the sampling volume was 10 L, the detection limit and quantitative limit of 25 aldehydes and ketones were less than 0.16 and 0.50 μg/m3 , respectively.