The use of bioaccumulation, biomarkers and histopathology diseases in shape Procambarus clarkii to establish bioavailability of Cd and Zn after a mining spill |
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Authors: | M L Martín-Díaz S R Tuberty C L McKenney Jr J Blasco C Sarasquete T A DelValls |
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Institution: | (1) Facultad de Ciencias del Mar y Ambientales, Campus Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain;(2) University of West Florida, Center for Environmental Diagnostics and Bioremediation, Pensacola, FL 32514, USA;(3) US Environmental Protection Agency NHEERL, Gulf Ecology Division, One Sabine Island Dr., Gulf Breeze, FL 32561, USA;(4) Instituto de Ciencias Marinas de Andalucía, Campus Río Pedro s/n, 11510 Puerto Real, Cádiz, Spain |
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Abstract: | Individuals of the crayfish Procambarus clarkii (males and females) were exposed simultaneously to cadmium and zinc during 21 days. Exposure concentrations were those determined
at the Guadiamar river after the Aznalcóllar mining spill (SW, Spain): 10 and 30 μg L−1 of cadmium and 1000 and 3000 μg L−1 of zinc. Three biomarkers (MT: metallothioneins like proteins, VTG: vitellogenin/vitellin like proteins and histopathology)
together with heavy metal bioaccumulation were determined in soft tissues of male and female P. clarkii. At the concentrations tested, increasing cadmium exposure resulted in increasing cadmium bioaccumulation and increasing subletal
effects (induction of MT, VTG and histopathological damage in tissues). Nevertheless, although increasing zinc exposure showed
increasing VTG induction and histopathological damages, not a positive relationship was determined with MT induction. Concerning
to responses determined in male and female crayfishes only differences were found between sexes at the highest cadmium exposure
concentration related to bioaccumulation in hepatopancreas tissues. Biomarkers responses to heavy metal contamination in this
crayfish, even VTG induction not before tested in heavy metal contamination assessment in crustaceans resulted potential tools
for the monitoring of heavy metal environmental contamination. |
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Keywords: | metallothionein vitellogenin reproduction heavy metals |
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