Toxicity Assessment of Treated Wastewater Using Cultured Human Cell Lines |
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| Authors: | H Narita J Abe N Funamizu T Takakuwa M Kunimoto |
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| Institution: | (1) Department of Environmental Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo 0608628, Japan;(2) Department of Public Health and Molecular Toxicology, School of Pharmaceutical Sciences, Kitasato University, 5-9-1, Shirogane, Minato-ku, Tokyo 1088641, Japan |
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| Abstract: | Bioassay using cultured human cell lines was applied to an effluent of a wastewater treatment plant (WWTP) in Sapporo to assess
their toxicity, and in order to investigate the fate of toxicity in the WWTP, bioassay of the water samples from several points
in WWTP (influent, effluent, return flow from thickener, from dewatering process and from incineration process) was performed.
We also applied bioassay to the mixture of the activated sludge from the investigated plant and artificial sewage. These results
showed that the toxicity of the effluent was more intensive than the influent, and organic matter released from activated
sludge bacteria during their decay process contributed to the increase of toxicity in the effluent. |
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| Keywords: | bioassay cultured human cell lines organic matter released from activated sludge bacterial cells toxicity assessment of treated wastewater wastewater reclamation |
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