Inhibition of erythrocyte delta-aminolevulinic acid dehydratase (ALAD) activity in fish from waters affected by lead smelters |
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Authors: | Schmitt Christopher J Caldwell Colleen A Olsen Bill Serdar Dave Coffey Mike |
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Institution: | (1) U.S. Geological Survey, Columbia Environmental Research Center, Columbia, Missouri, U.S.A.;(2) U.S. Geological Survey, New Mexico Cooperative Fish and Wildlife Research Unit, Las Cruces, New Mexico, U.S.A;(3) U.S. Fish and Wildlife Service, Montana Field Office, Helena, Montana, U.S.A;(4) Washington Department of Ecology, Environmental Investigations and Laboratory Services Program, Olympia, Washington, U.S.A;(5) U.S. Fish and Wildlife Service, Rock Island Field Office, Rock Island, Illinois, U.S.A |
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Abstract: | We assessed the effects on fish of lead (Pb) released to streamsby smelters located in Trail, BC (Canada), E. Helena, MT, Herculaneum, MO, and Glover, MO. Fish were collected by electrofishing from sites located downstream of smelters and from reference sites. Blood from each fish was analyzed for -aminolevulinic acid dehydratase (ALAD) activity and hemoglobin (Hb), and samples of blood, liver, or carcass were analyzed for Pb, zinc (Zn), or both. Fish collected downstreamof all four smelters sites had elevated Pb concentrations, decreased ALAD activity, or both relative to their respectivereference sites. At E. Helena, fish from the downstream site also had lower Hb concentrations than fish from upstream. Differences among taxa were also apparent. Consistent with previous studies, ALAD activity in catostomids (Pisces: Catostomidae-northern hog sucker, Hypentelium nigricans;river carpsucker, Carpiodes carpio; largescale sucker, Catostomus macrocheilus; and mountain sucker, C. platyrhynchus) seemed more sensitive to Pb-induced ALAD inhibition than the salmonids (Pisces: Salmonidae-rainbow trout,Oncorhynchus mykiss; brook trout, Salvelinus fontinalis) or common carp (Cyprinus carpio). Some of these differences may have resulted from differential accumulation of Zn, which was not measured at all sites. We detected no ALAD activity in channel catfish (Ictaluruspunctatus) from either site on the Mississippi River at Herculaneum, MO. Our findings confirmed that Pb is releasedto aquatic ecosystems by smelters and accumulated by fish, andwe documented potentially adverse effects of Pb in fish. We recommend that Zn be measured along with Pb when ALAD activityis used as a biomarker and the collection of at least 10 fish ofa species at each site to facilitate statistical analysis. |
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Keywords: | ALAD Columbia River fish hemoglobin lead metals Mississippi River smelters zinc |
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