Isolation and Identification of Enterococci from Seawater Samples: Assessment of Their Resistance to Antibiotics and Heavy Metals

A hundred Enterococcus strains were isolated from seawater samples collected from coastal areas of Istanbul. Isolates were identified to the species level using standard biochemical tests specified by Facklam and Collins. The species distribution was as follows Enterococcus faecalis (96%), Enterococcus gallinarum (3%) and Enterococcus solitarius (1%). The resistance of bacteria to both heavy metals (zinc Zn], iron Fe], cadmium Cd], chrome Cr], cobalt Co]) and antibiotics (ampicillin 10 μg AP], penicillin G 10 Units PG], gentamycin 10 μg GM], streptomycin 10 μg S], chloramphenicol 10 μg C], erythromycin 15 μg E], kanamycin 30 μg K], amikacin 30 μg AK], nalidixic acid 30 μg NA], and vancomycin 30 μg VA]) was evaluated. None of the strains was resistant to VA. It was found that among the 100 isolates, those that exhibit resistance to antibiotics, particularly NA, S and K, were also resistant all the heavy metals tested. To our knowledge this is the first report focusing on determination of resistance of environmental enterococci found in Istanbul against heavy metals and antibiotics. Thus, combined expressions of antibiotic and heavy metal resistance may help to reinforce ecological and epidemiological studies and to determine the role of these strains in antibiotic and heavy metal resistance dissemination.