PCR技术对水中病原体的检测

水中有病毒、细菌、原生动物、蠕虫等多种病原体。常规的检测方法是用大肠菌作为病原体的指示生物,但是大肠菌的生存特性与病毒、原生动物差异较大,难以指示病毒、原生动物的存在与否。对每一种病原体单独进行检测,费时、费力。由于PCR技术具有快速、灵敏、特异的特点,使得它对于水中病原体的检测具有较高的应用价值。

Application of PCR in the monitoring of pathogen in the water

The pathogen in the waters are viruses, bacteria, protozoa and helminths. The conventional method is monitoring the indicator bacteria E.coli. Because the surviving character of E.coli is quite different from the virus and protozoa, it is difficult for it to indicate the presence of pathogens. While the monitoring of different pathogens using the conventional manner is time consuming and tedious, the rapid , sensitive and accurate PCR technique make it possible to replace the conventional method to monitor the pathogen in the water on routine basis.