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地表水浮游植物叶绿素a测定方法比较研究
引用本文:翁笑艳,林美爱,严颖.地表水浮游植物叶绿素a测定方法比较研究[J].中国环境监测,2009,25(3).
作者姓名:翁笑艳  林美爱  严颖
作者单位:福州市环境监测中心站,福建,福州,350011
摘    要:比较了浮游植物叶绿素a测定方法中的丙酮萃取法和热乙醇萃取法.结果表明,应用反复冻融植物细胞的丙酮萃取法对地表水中浮游植物叶绿素a的萃取率显著高于其他方法,且重复测定的精密度较高.

关 键 词:地表水  浮游植物  叶绿素a  丙酮  热乙醇

Comparison of Determination of Phytoplankton Chlorophyll a by Spectroscopic Methods in Freshwater
WENG Xiao-yan,et al.Comparison of Determination of Phytoplankton Chlorophyll a by Spectroscopic Methods in Freshwater[J].Environmental Monitoring in China,2009,25(3).
Authors:WENG Xiao-yan  
Institution:Fuzhou Environmental Monitoring Centre;Fuzhou 350011;China
Abstract:Chlorophyll a is the principal pigment in plants. As a biomass indicator of aquatic micro-algae,it is probably the most frequently measured biochemical parameter in freshwater.Whatever the method used for the determination of algal pigments,the measure itself relies on their spectroscopic characteristics: light absorption or fluorescence.In discrete samples,photosynthetic pigments can be measured either by the traditional non-separative spectroscopic methods or after chromatographic separation,using HPLC.Th...
Keywords:Freshwater  Phytoplankton  Chlorophyll a  Acetone  ethanol  
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