| 荧光定量PCR技术在环境领域的应用 |
| |
| 引用本文： | 魏春花,曾文炉,周启星,谢秀杰.荧光定量PCR技术在环境领域的应用[J].中国环境监测,2012,28(4):48-53. |
| |
| 作者姓名： | 魏春花 曾文炉 周启星 谢秀杰 |
| |
| 作者单位： | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津 300071;南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津 300071;南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津 300071;中国科学院沈阳应用生态研究所, 中国科学院陆地生态过程重点实验室, 辽宁 沈阳 110016;南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津 300071 |
| |
| 基金项目： | 国家自然科学基金项目(20977053,21077059) |
| |
| 摘 要： | 实时荧光定量PCR技术作为一项新兴技术,越来越受到人们的重视,它以快速、准确定量、便捷等优点广泛应用于科学研究各个领域。近年来,实时荧光定量PCR技术不断发展,并应用于环境领域中,大大提高了环境监测水平。综述了实时荧光定量PCR技术的基本原理及其在国内外环境领域中的应用。
|
| 关 键 词： | 荧光定量PCR 荧光探针 环境监测 |
| 收稿时间： | 2010/12/10 0:00:00 |
| 修稿时间： | 4/7/2011 12:00:00 AM |
Application of Real-time Fluorescent Quantitative PCR in Environmental Area |
| |
| WEI Chun-hu,ZENG Wen-lu,ZHOU Qi-xing.Application of Real-time Fluorescent Quantitative PCR in Environmental Area[J].Environmental Monitoring in China,2012,28(4):48-53. |
| |
| Authors: | WEI Chun-hu ZENG Wen-lu ZHOU Qi-xing |
| |
| Institution: | Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China;Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China;Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China;Key Laboratory of Terrestrial Ecological, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China |
| |
| Abstract: | As an emerging technology, Real-time fluorescence quantitative PCR technology draws more and more attention from scienctists. Since it is fast, accurate and easy use, It is widely used in scientific research fields. In recent years, with the developing and more detailed of the technology, it is applied in environmental area and has greatly improved the efficiency of detection in environment monitoring. This paper reviewed the quantitative fluorescence detection of PCR basic principle and its application in environmental fields at home and abroad. |
| |
| Keywords: | Real-time fluorescent quantitative PCR Fluorescent probe Environment monitoring |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国环境监测》浏览原始摘要信息 |
| 点击此处可从《中国环境监测》下载免费的PDF全文 |
|
