基于磁性分离测定水样中的吲哚美辛

免疫磁性微球(SMPPA)应用于水样中吲哚美辛的分离富集,并应用高效液相色谱法对吲哚美辛进行测定。在优化的淋洗和洗脱条件下,70%甲醇和10%甲醇分别作为洗脱液和淋洗液,其回收率能达到90%。选择了湖水、河水、自来水、污水厂进口水以及医院排水为真实样品进行测定,其中湖水、河水、自来水3种真实水样中未能检测出吲哚美辛,污水厂进口水以及医院排水中吲哚美辛的含量分别为0.762、35.4 ng/m L。对这5种水进行加标回收吲哚美辛,加标回收率为92.7%~113%,RSD为1.09%~7.73%。该方法具有简便、快速、特异性等特点,能有效地分离富集水样中的目标物,对其他目标物具有广泛的实际应用价值。

Detection of Indomethacin in Water Sample Based on Magnetic Separation

Immunonogic magnetic microsphere was used to separate indomethac(IDM) from water sample. Under the optimized conditions, 70% of methanol and 10% of methanol were selected as eluant and washing solution respectively. Lake water, river water, tap water, imported water to sewage plant, drainage of hospital were choose as real sample. Indomethacin was not detected in lake water, river water and tap water,but 0.762 ng/mL and 35.4ng/mL in imported water to sewage plant and drainage of hospital. Recovery tests on this five kinds of water sample. The recovery rate of five kinds of spiked water samples was found to be 92.7%-113% with the relative standard deviations (RSD) of 1.09%-7.73%. This method is simple, fast and specificity for the separation and enrichment of target analyte(indomycin) from water samples. This separation technology would be applied for other compounds.