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不同碳源及光照对小球藻生长和产油脂的影响
引用本文:刘香华,刘雷,曾慧卿.不同碳源及光照对小球藻生长和产油脂的影响[J].安全与环境学报,2012,12(3):6-10.
作者姓名:刘香华  刘雷  曾慧卿
作者单位:南昌大学环境与化学工程学院,南昌,330031;南昌大学环境与化学工程学院,南昌,330031;南昌大学环境与化学工程学院,南昌,330031
摘    要:考察了3种碳源(葡萄糖、NaHCO3和乙酸钠)在不同初始质量浓度和光照条件下对小球藻(Chlorella vulgaris)生长和产油脂的影响。采用OD680和生物量来评价小球藻的生长情况;以溶剂浸提法提取生物油脂,并以油脂质量分数和油脂产量来描述产油脂特性。结果表明,经过9 d的培养,3种碳源中葡萄糖是最佳有机碳源。由其培养的小球藻生长速率最快,由NaHCO3培养的小球藻的生长效果不如葡萄糖,而乙酸钠不利于小球藻的生长。随着光照的增强,小球藻光合效率提高,生物量逐渐提高,5 000 lx最利于小球藻的生长,而1 600 lx最利于小球藻油脂的积累。研究表明,光照5 000 lx下,初始质量浓度为15 g/L的葡萄糖作为碳源是小球藻适宜的生长和产油条件,获得了3.17 g/L的最大生物量和1.025 g/L的最大油脂产量。

关 键 词:环境工程学  小球藻  碳源  光照条件  微藻油脂

Effects of different carbon sources and light intensities on the growth and the lipid properties of Chlorella vulgaris
LIU Xiang-hua , LIU Lei , ZENG Hui-qing.Effects of different carbon sources and light intensities on the growth and the lipid properties of Chlorella vulgaris[J].Journal of Safety and Environment,2012,12(3):6-10.
Authors:LIU Xiang-hua  LIU Lei  ZENG Hui-qing
Institution:(College of Environmental and Chemical Engineering,Nanchang University,Nanchang 330031,China)
Abstract:The essay is inclined to present our experimental results of the effects of the three carbon sources,i.e.glucose,NaHCO3 and sodium acetate.We have also studied the effects of their respective initial concentrations on the growth and lipid properties of Chlorella vulgaris at different light intensities.In our experiments,we have also used OD680 and biomass for evaluation of the growth of Chlorella vulgaris,and extracted the microalgae oil with the solvent extraction method.In addition,the lipid properties and the percentage of lipid and lipid production were used for the lipid properties.The results of our experiments demonstrate that,after nine-day cultivation,of the three carbon sources,glucose proves to be the best organic carbon source.When glucose was added as the carbon source,Chlorella vulgaris tends to grow fatter than the other two.When NaHCO3 was used as the carbon source,the growth rate tends to be too slow,moreover,no great help would there be found to promote the growth of Chlorella vulgaris.What is more,if the initial concentration of NaHCO3 was too high,it might inhibit the growth of Chlorella vulgaris.And,then,when sodium acetate was added as the carbon source,it wouldn’t make any obvious help to the growth of Chlorella vulgari,neither could it be the optimal carbon source for the growth of Chlorella vulgaris.Furthermore,under the three light intensities as was mentioned above,when the light intensity was increased,the photosynthetic efficiency of Chlorella vulgaris tends to improve gradually.And,when the light intensity was set at 5 000 lx,Chlorella vulgaris began to grow faster,and,then,its biomass would grow much faster.Thus,it can be concluded that 5 000 lx should be the optimal light intensity for the growth of Chlorella vulgaris.Therefore,of the three light intensities,1 600 lx proves to be much more beneficial for the lipid accumulation of Chlorella vulgaris than the other two.And,last of all,the above experiments make us realize that the best condition of the production of lipid was: the light intensity of 5 000 lx;the carbon source is the best source to be chosen with the initial mass concentration being 15 g/L.The peak biomass should be chosen as 3.17 g/L with the peak lipid production of 1.025 g/L.
Keywords:environmental engineering  Chlorella vulgaris  carbon source  light condition  microalgae oil
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