鼠李糖脂、Fe3+及Mg2+对蜡质芽孢杆菌DL-1降解邻二氯苯的复合影响

以蜡质芽孢杆菌DL-1降解邻二氯苯,研究不同浓度的鼠李糖脂、Fe³⁺、Mg²⁺对邻二氯苯生物降解效果的影响。通过响应面优化法获得最适加入量,并考察添加鼠李糖脂、Fe³⁺、Mg²⁺的生物滴滤塔中邻二氯苯废气的处理效果。实验结果表明：鼠李糖脂、Fe³⁺、Mg²⁺的最适加入量分别为120,4.0,2.0 mg/L;在最适加入量下培养48 h后,邻二氯苯降解菌DL-1能够保持菌体完整,培养72 h后邻二氯苯去除率可达98.3%;在鼠李糖脂、Fe³⁺、Mg²⁺加入量为最适加入量、空床停留时间为60 s、进气中邻二氯苯质量浓度为1 000 mg/m³时,生物滴滤塔对邻二氯苯的去除率达到90.0%。

Composite effects of rhamnolipid,Fe3+ and Mg2+ on degradation of o-dichlorobenzene by Bacillus cereus strain DL-1

o-Dichlorobenzeneas was degraded by a strain of Bacillus cereus DL-1. The effects of rhamnolipid,Fe³⁺ and Mg²⁺ with different concentration on the bio-degradation were studied. Their optimum conditions were obtained by using response surface optimization method,and the treatment effect of o-dichlorobenzene-containing waste gas in a biotrickling filter with rhamnolipid,Fe³⁺ and Mg²⁺ addition were investigated. The experimental results show that：The optimum dosage of rhamnolipid,Fe³⁺,and Mg²⁺ is 120 ,4.0,2.0 mg/L respectively;Under the optimum dosages,the Bacillus cereus DL-1 strain can keep intact after cultured for 48 h,and the o-dichlorobenzene removal rate is 98.3% after cultured for 72 h;When rhamnolipid,Fe³⁺,and Mg²⁺ are added with the optimum dosage,the empty bed residence time is 60 s and the mass concentration of o-dichlorobenzene in the biotrickling filter inlet is 1 000 mg/m³,the o-dichlorobenzene removal rate is 90.0%.