黑麦草-不动杆菌组合体系对石油污染土壤的生物强化修复

以盆栽实验为基础，研究了植物（黑麦草，Lolium perenne L）-微生物（不动杆菌，Acinetobacter sp.）组合体系对石油污染土壤的修复效果。实验结果表明：在总石油烃含量为4 420.18 mg/kg、脱氢酶活性为230.52 μg/（g·d）、苯酚毒性当量浓度（TEQphenol）为1 633.21 mg/L的初始条件下，强化组总石油烃降解率最高为53.08%，是对照组的1.60倍；土壤的脱氢酶活性达到637.73 μg/（g·d），是对照组的10.64倍；石油污染土壤的生物毒性大幅降低， TEQphenol最终降低至171.08 mg/L。说明该组合体系对石油污染土壤具有很好的修复作用，且微生物对土壤中有毒物质的降解起主要作用。

Enhanced bioremediation of petroleum-contaminated soil using ryegrass-acinetobacter combination system

The bioremediation effects of petroleum-contaminated soil were studied using a plant（ryegrass，Lolium perenne L）-microbe（acinetobacter，Acinetobacter sp.） combination system with pot culture. The experimental results showed that under the initial conditions of total petroleum hydrocarbon content 4 420.18 mg/kg，dehydrogenase activity 230.52 μg/（g·d） and toxic equivalent quangtity（ converted to toxic equivalent quangtity of phenol，TEQphenol） 1 633.21 mg/L，the highest degradation rate of total petroleum hydrocarbon of the enhancement group was 53.08% which was 1.60 times higher than that of the blank group，the dehydrogenase activity of the soil reached 637.73 μg/（g·d） which was 10.64 times higher than that of the blank group，and the biotoxicity of the contaminated soil was significantly reduced with the lowest TEQphenol of 171.08 mg/L. These results indicated that this combination system had good effects on bioremediation of petroleum-contaminated soil，and microbes played a major role in degradation of toxic substances in soil.