Production of 2-Pyrrolidone from Biobased Glutamate by Using Escherichia coli |
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Authors: | Naoko Yamano Norioki Kawasaki Sahori Takeda Atsuyoshi Nakayama |
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Institution: | 1. Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Osaka, 563-8577, Japan 2. Research Institute for Ubiquitous Energy Devices, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Osaka, 563-8577, Japan
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Abstract: | We have developed a simple and highly efficient process for the production of 2-pyrrolidone (2-PRN) from biobased l-glutamic acid (Glu). First, we produced γ-aminobutyric acid (GABA) from Glu obtained by fermentation of biomass using Escherichia coli, which is known to possess GABA producing activity. The reaction solution contained only the substrate Glu, bacterial cells, and water, and did not require buffers or coenzymes, pyridoxal-5′-phosphate (PLP). Every 24 h, cells were removed by centrifugation, and GABA containing supernatant was obtained. This reaction can be repeated 14 times by adding water and Glu, without any decrease in activity. Finally, 303.7 g of GABA was produced from 560 g (40 g × 14 times) of Glu with a yield of 77.4 %. The concentration of this solution was almost 40 %. The GABA was then converted to biobased 2-PRN by heating and distillation under reduced pressure without pretreatment. The yield obtained with this chemical process was 95.8 %. These results showed that biobased 2-PRN could be produced from biomass-derived Glu. Biobased 2-PRN has great potential as a raw material to change other petroleum-based materials to biobased materials. |
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